| 引用本文:王焱冰1，刘炳君1，刘缨1，2.不同温度下短时间磁场处理对大鼠血清的影响[J].世界中医药,2022,(24):. |
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| 不同温度下短时间磁场处理对大鼠血清的影响 |
| Effect of Short-time Magnetic Field Treatment at Different Temperatures on Serum-related Indexes in Rats |
| 投稿时间：2022-10-17 |
| DOI：10.3969/j.issn.1673-7202.2022.24.009 |
| 中文关键词: 血液保存 磁场 温度 大鼠 谷草转氨酶 谷丙转氨酶 超氧化物歧化酶 2,3-二磷酸甘油酸 |
| English Keywords:Blood preservation Magnetic field Temperature Rat Glutamic-oxaloacetic transaminase Glutamic-pyruvic transaminase Superoxide dismutase 2,3-diphosphoglycerate |
| 基金项目:国家自然科学基金项目（31870840） |
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| 中文摘要: |
| 目的：研究在不同温度下不同磁场强度对体外血液的影响，为不同磁场对血液影响的研究提供参考思路。方法：大鼠随机分为室温组（25 ℃）和低温组（0~4 ℃），每个温度组又分别设置地磁场组（GMF）、亚磁场组（HMF）、强磁场组（SMF）和超强磁场组（ESMF）。处理2 h后，立即取血清，对其中的谷草转氨酶（GOT）、谷丙转氨酶（GPT）、超氧化物歧化酶（SOD）和2,3-二磷酸甘油酸（2,3-DPG）的含量进行检测。结果：短时间（2 h）室温下ESMF能够使GOT活性显著下降（P<0.01），GPT活性出现下降趋势；低温环境或许能加强各磁场对GOT和GPT的效应；室温下HMF、SMF和ESMF以及低温下HMF和SMF都使2,3-DPG出现上升趋势，低温环境下ESMF能够使2,3-DPG显著降低(P<0.005）；不同温度下各磁场对SOD活性暂无影响。结论：短时间亚磁场将保护体外血液蛋白酶活性，同时，血样运输中应避免超强磁场的负面影响。为揭示磁场效应机制以及临床和科研中血样的保存技术开发提供了必要的基础依据和线索，具有一定的理论意义和应用价值。 |
| English Summary: |
| To analyze the effect of different magnetic field strengths on blood in vitro at different temperatures and provide ideas for the research on the effect of different magnetic fields on blood.Methods:Rats were randomly divided into a room temperature group(25 ℃) and a low temperature group(0-4 ℃).In each group，geomagnetic group(GMF)，hypomagnetic field group(HMF)，strong magnetic group(SMF)，and extremely strong magnetic field group(ESMF) were set up.Immediately after 2 h of treatment，serum samples were collected and the levels of glutamic-oxaloacetic transaminase(GOT)，glutamic-pyruvic transaminase(GPT)，superoxide dismutase(SOD)，and 2,3-diphosphoglycerate(2,3-DPG) were measured.Results:ESMF at room temperature for a short time(2 h) was able to cause a significant decrease in GOT activity(P<0.01) and a downward trend in GPT activity.The low temperature might enhance the effect of each magnetic field on GOT and GPT.HMF，SMF，and ESMF at room temperature and HMF and SMF at low temperature caused an upward trend in 2,3-DPG，and ESMF at low temperature was able to significantly lower 2,3-DPG(P<0.005).There was no effect of each magnetic field on SOD at different temperatures.Conclusion:HMF can protect the in vitro blood protease activity in a short time，and the negative effect of ESMF should be avoided in the transport of blood samples.The findings of this study are expected to provide a necessary basis and references for revealing the mechanism of the magnetic field effect and the technological development of blood sample preservation in clinical and scientific research，which has certain theoretical significance and application value. |
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