| 引用本文:李克建1，李静1，李红2.补阴益智汤配方颗粒对睡眠剥夺小鼠记忆力及睡眠质量的影响[J].世界中医药,2022,(24):. |
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| 补阴益智汤配方颗粒对睡眠剥夺小鼠记忆力及睡眠质量的影响 |
| Effects of Buyin Yizhi Decoction Dispensing Granules on Memory and Sleep Quality of Sleep-deprived Mice |
| 投稿时间：2020-11-25 |
| DOI：10.3969/j.issn.1673-7202.2022.24.011 |
| 中文关键词: 补阴益智汤配方颗粒 睡眠剥夺 睡眠质量 记忆力 记忆通路 神经再生通路 神经递质 小鼠 |
| English Keywords:Buyin Yizhi Decoction Dispensing Granules Sleep deprivation Sleep quality Memory Memory pathway Nerve regeneration pathway Neurotransmitter Mouse |
| 基金项目:国家自然科学基金项目（81973921）——江南卷柏黄酮调控NLRP3炎症小体抑制细胞焦亡治疗痛风的作用机制 |
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| 中文摘要: |
| 目的：分析补阴益智汤配方颗粒（BYT）对睡眠剥夺（SD）小鼠记忆力及睡眠质量的影响，并探讨其机制。方法：选取ICR小鼠60只，用随机数字表法分为正常对照组、模型组、BYT低剂量组（6 g/kg）、BYT中剂量组（12 g/kg）、BYT高剂量组（18 g/kg）、阳性对照组（0.9 mg/kg）组，每组10只，建立长期SD模型。检测学习记忆能力、神经递质水平、睡眠质量、海马神经元细胞凋亡率；蛋白质印迹法测学习记忆通路-蛋白激酶A-催化亚基β（PKA-Cβ）/cAMP反应元件结合蛋白（CREB）/原肌球蛋白相关激酶B（TrkB）、神经再生通路-配体蛋白（Wnt）/β-连环素/β-半乳糖苷酶（β-gal）蛋白表达。结果：与正常对照组比较，模型组小鼠学习记忆能力及睡眠质量降低、神经递质释放异常、海马神经元凋亡率升高、记忆通路PKA-Cβ/CREB/TrkB及神经再生通路Wnt/β-连环素/β-gal蛋白表达降低（P<0.05）。与模型组比较，BYT低剂量组、BYT中剂量组、BYT高剂量组及阳性对照组小鼠学习记忆能力及睡眠质量升高、神经递质释放趋于正常，神经元细胞凋亡率降低，学习记忆及神经再生通路蛋白表达升高（P<0.05）。结论：BYT可调节神经递质释放，促进记忆及神经再生通路激活，改善SD小鼠睡眠质量及学习记忆能力。 |
| English Summary: |
| To analyze the effects of Buyin Yizhi Decoction Dispensing Granules(BYD) on memory and sleep quality of sleep-deprived(SD) mice and explore the underlying mechanism.Methods:Sixty ICR mice were randomly divided into a normal control group，a model group，low-，medium-，and high-dose BYD groups(6，12，and 18 g/kg)，and a positive control group(0.9 mg/kg)，with 10 mice in each group.The long-term SD model was established.The learning and memory ability，neurotransmitter level，sleep quality，and apoptosis rate of hippocampal neurons were detected.The protein expression of learning and memory pathway kinase A-Cβ subunit(PKA-Cβ)/cyclic AMP response element-binding protein(CREB)/tropomyosin receptor kinase B(TrkB) and nerve regeneration pathway ligand protein(Wnt)/β-catenin/β-galactosidase(β-gal) was detected by Western blot.Results:Compared with the normal control group，the model group showed reduced learning and memory ability and sleep quality，abnormal release of neurotransmitters，increased apoptosis rate of hippocampal neurons，and decreased protein expression of memory pathway PKA-Cβ/CREB/TrkB and nerve regeneration pathway Wnt/β-catenin/β-gal(P<0.05).Compared with the model group，the BYD groups and the positive control group showed increased learning and memory ability and sleep quality，normalized neurotransmitter release，decreased apoptosis rate of hippocampal neurons，and increased protein expression of learning，memory and nerve regeneration pathways(P<0.05).Conclusion:BYD can regulate the release of neurotransmitters，promote the activation of memory and nerve regeneration pathways，and improve the sleep quality and learning and memory ability of SD mice. |
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