世界中医药

目的：通过观察固本健脑液对阿尔茨海默病（AD）模型大鼠学习记忆、睡眠持续时间、行为次数以及睡眠-觉醒时相的影响，探索固本健脑液可能的调节机制。方法：将实验大鼠随机分成空白组、模型组、固本健脑液组以及褪黑素（MT）组4组，每组10只。HomecageScan3.0实时监测系统检测各组模型大鼠在笼内的行为活动情况并进行24 h分析，采用Morris水迷宫检测各组大鼠学习及空间记忆能力；使用Sleepsign3.0进行24 h脑电肌电描记。结果：Morris水迷宫结果显示，模型组较空白组大鼠逃避潜伏期延长，找到目标平台区域的游泳时间百分比下降（P<0.05）；固本健脑液组以及MT组逃避潜伏期明显减少，找到目标平台区域的游泳时间百分比显著上升（P<0.05）；HomeCage监测显示，与空白组比较，模型组觉醒次数多且昼夜节律颠倒，睡眠持续时间降低（P<0.05），干预后，固本健脑液组觉醒次数减少且昼夜节律正常，睡眠持续时间延长；Sleepsign脑电肌电描记结果显示：模型组较空白组快速眼球运动（REM）睡眠累积量降低（P<0.05），非快速眼球运动（NREM）睡眠降低（P<0.05）；干预后，固本健脑液组较空白组觉醒次数减少，NREM睡眠增多（P<0.05）。结论：AD模型大鼠存在一定程度的昼夜节律紊乱，而使用固本健脑液干预后可改善AD大鼠的昼夜节律紊乱，提高AD大鼠的睡眠质量，使睡眠节律恢复趋于正常。

English Summary:

To observe the effects of Guben Jiannao Liquid(GBJNL) on learning and memory，sleep duration，number of behaviors and sleep-wake phase in Alzheimer′s Disease(AD) model rats，to explore the possible regulatory mechanism of GBJNL.Methods:The experimental rats were randomly divided into blank group，model group，GBJNL group and melatonin(MT) group，with 10 in each group.HomeCageScan 3.0 was used to detect and analyze the behaviors of the modeled rats in the cage for 24 h.Morris water maze was used to test the learning and spatial memory ability of rats in each group.The electroencephalography and electromyography were performed for 24 h using Sleepsign 3.0.Results:Morris water maze showed that compared with the conditions in the blank group，the escape latency of rats in the model group was prolonged，and the percentage of swimming time in target quadrant was decreased(P<0.05).Compared with the model group，the GBJNL group and the MT group had shortened escape latency and increased percentage of swimming time in target quadrant(P<0.05).HomeCageScan monitoring revealed that the model group had higher number of awakenings with reversed circadian rhythm，and lower sleep duration than the blank group(P<0.05)，and after intervention，the number of awakenings in the GBJNL group was reduced with normal circadian rhythms，and the sleep duration was prolonged.The electroencephalography and electromyography by Sleepsign indicated that compared with the blank group，the model group had decreased accumulation of rapid eye movement(REM) sleep(P<0.05) and non-rapid eye movement(NREM) sleep(P<0.05)，and after intervention，the number of awakenings in the GBJNL group was lower while the accumulation of NREM sleep was higher than those in the blank group(P<0.05).Conclusion:The AD model rats have a certain degree of circadian rhythm disorders，and GBJNL can improve the circadian rhythm disorders in them，increase the sleep quality and promote the sleep rhythm back to normal.

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