To observe the changes in brain functional connectivity(FC) between the dorsal premotor cortex(PMd)，the ventral premotor cortex(PMv)，and the primary motor cortex(M1) after different degrees of motor function injury based on functional magnetic resonance imaging(fMRI) technology，explore the effect mechanism and sites of brain acupuncture after stroke-induced hemiplegia，and provide imaging evidence for acupuncture to promote the remodeling of brain function after stroke-induced hemiplegia.Methods:Twenty-six cases of patients with left hemiplegia admitted to the Dongzhimen Hospital，Beijing University of Chinese Medicine from September 2014 to September 2019 were enrolled as research subjects，including 18 mild and moderate cases(50-99 points) and eight severe cases(0-49 points) according to Fugl-Meyer assessment(FMA).The patients underwent clinical scale evaluation and fMRI scanning.The bilateral PMd，PMv，and M1 were selected as seed points，and the changes in functional connectivity between the 15 pairs of seed points and the correlation with FMA were compared.Results:In all the groups，the FC between the contralesional PMd-affected M1，bilateral PMv，and bilateral PMd under acupuncture was significantly higher than that under the resting state(P<0.05).The FC between contralesional PMd-affected M1，affected PMD-M1，and bilateral M1 in the mild and moderate group under acupuncture was higher than that under the resting state(P<0.05)，and the FC between affected PMv-M1 in the severe group under acupuncture was lower than that under the resting state(P<0.05).The FC between affected PMv-contralesional M1 and affected PMv-M1 in the severe group under the resting state was higher than that in the mild to moderate group(P<0.05).In the severe group，FMA was positively correlated with FC between contralesional PMv-affected M1，contralesional PMv-affected PMd，and bilateral PMv under the resting state(r>0，P<0.05).FMA was positively correlated with FC between contralesional PMd-affected M1 and affected PMd-M1 under acupuncture(r>0，P<0.05).Conclusion:After different degrees of motor function impairment induced by cerebral infarction，there are corresponding brain areas in brain function remodeling，and acupuncture on Yanglingquan can promote the recovery of motor function and affect the recovery and remodeling of brain function after stroke-induced hemiplegia by selecting specific effect points.