| 引用本文:刘金莲1，吕美豫1，梁唯灿1，邸松蕊1，南海鹏1，王林元1，张建军1，赵晋燕2，曹春然2，王淳1.基于证效相关研究清利湿热方中医功效及降血尿酸作用机制[J].世界中医药,2023,(01):. |
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| 基于证效相关研究清利湿热方中医功效及降血尿酸作用机制 |
| Traditional Chinese Medicine Efficacy of Qingli Shire Prescription and Its Mechanism of Lowering Blood Uric Acid Based on Syndrome-efficacy Study |
| 投稿时间：2022-11-15 |
| DOI：10.3969/j.issn.1673-7202.2023.01.007 |
| 中文关键词: 清利湿热方 湿热内蕴 证候模型 高尿酸血症 |
| English Keywords:Qingli Shire Prescription Internal retention of dampness-heat Syndrome model Hyperuricemia |
| 基金项目:国家重点研发计划（2018YFC1706803） |
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| 中文摘要: |
| 目的：建立湿热内蕴证候大鼠模型，研究具有降血尿酸作用的清利湿热方的中医功效，以证测效揭示清利湿热降血尿酸作用机制。方法：将48只SD大鼠随机分为空白组、模型组、四妙丸组以及清利湿热方低、中、高剂量组，每组8只。采用饲喂高脂高糖饲料，自由饮用蜂蜜水，并隔天灌胃猪油脂或酒，建立湿热内蕴证候大鼠模型，观察清利湿热方对大鼠一般状态的影响。采用酶联免疫吸附试验检测血清热激蛋白70（HSP70）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β），促胃液素（GAS）、免疫球蛋白G（IgG）、免疫球蛋白M（IgM）含量以及血浆胃动素（MTL）、肝脏组织Na+-K+-ATP酶含量，免疫组织化学法检测胃肠组织水孔蛋白3（AQP3）、水孔蛋白4（AQP4）含量，采用蛋白质印迹法检测结肠组织中TOLL样受体4(TLR4)、核因子κBp65表达量。结果：模型组体质量下降（P<0.05，P<0.01），HSP70、TNF-α、IL-1β升高（P<0.05，P<0.01，P<0.001），胃肠组织中AQP3表达量下降（P<0.05），AQP4表达量上升（P<0.01，P<0.001），GAS含量下降（P<0.01），IgM含量升高（P<0.01），结肠组织TLR4表达量增加（P<0.01）。清利湿热方能增加大鼠体质量，降低HSP70、TNF-α、IL-1β含量（P<0.05，P<0.01，P<0.001），升高胃肠组织AQP3表达量（P<0.05，P<0.01，P<0.001），降低AQP4表达量（P<0.05），升高GAS、Na+-K+-ATP酶含量（P<0.05，P<0.001），降低IgM含量（P<0.05，P<0.01）。结论：饲喂高脂高糖饲料，自由饮用蜂蜜水，并隔天灌胃油脂/酒，可以成功建立高尿酸血症湿热内蕴证候模型，清利湿热方可以从大鼠一般状态、体质量、热激蛋白以及炎症介质、水孔蛋白、胃肠激素、免疫等多方面改善大鼠湿热状态，发挥清利湿热功效。清利湿热方具有降血尿酸作用，其作用机制可能与下调TLR4，核因子κBp65有关。 |
| English Summary: |
| To study the traditional Chinese medicine(TCM) efficacy of Qingli Shire Prescription(QLSR) by establishing a rat model of internal retention of dampness-heat，to reveal its mechanism of lowing blood uric acid based on testing efficacy by syndrome.Methods:A total of 48 SD rats were randomized into blank group，model group，Simiao Pills group and QLSR low-，medium-and high-dose groups，with 8 in each group.A rat model of internal retention of dampness-heat was established by high-fat and high-sugar diet+free honey water+lard/alcohol every other day(ig).The general state of rats was observed.The serum heat shock protein 70(HSP70)，tumor necrosis factor-α(TNF-α)，interleukin-1β(IL-1β)，gastrin(GAS)，immunoglobulin G(IgG)，immunoglobulin M(IgM)，plasma motilin(MTL) and Na+-K+-ATP in liver tissue were detected by enzyme-linked immunosorbent assay.The contents of aquaporin 3(AQP3) and aquaporin 4(AQP4) in gastrointestinal tissues were determined by immunohistochemistry.Western blot was used to detect the expressions of Toll-like receptor 4(TLR4) and nuclear factor-kappa B(NF-κB) p65 in colon tissues.Results:In the model group，body weight loss was observed(P<0.05，P<0.01); the levels of HSP70，TNF-α and IL-1β were increased(P<0.05，P<0.01，P<0.001); the expression of AQP3 was down-regulated(P<0.05)，while the expression of AQP4 was up-regulated(P<0.01，P<0.001); the content of GAS was lowered(P<0.01)，while the content of IgM was elevated(P<0.01); an increase was found in the expression of TLR4(P<0.01).QLSR increased the body weight of rats，down-regulated the levels of HSP70，TNF-α and IL-1β(P<0.05，P<0.01，P<0.001)，the expression of AQP4(P<0.05) and the content of IgM(P<0.05，P<0.01) and up-regulated the expression of AQP3(P<0.05，P<0.01，P<0.001) and the contents of GAS and Na+-K+-ATP(P<0.05，P<0.001).Conclusion:The syndrome model suitable for evaluating the internal retention of dampness-heat of hyperuricemia can be successfully established by high-fat and high-sugar diet+free honey water+lard/alcohol every other day(ig).QLSR improves the dampness-heat state of rats from the general state，body weight，heat shock protein，inflammatory factors，aquaporins，gastrointestinal hormones，immunity and other aspects，and exerts the effect of clearing dampness-heat.The prescription lowers blood uric acid，and its mechanism may be related to the down-regulation of TLR4 and NF-κB p65. |
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