| 引用本文:刘晶，黄文娟，卢军，张彦，范芳芳，赵生俊.基于2型糖尿病小鼠模型探索生品黑豆、淡豆豉对二肽基肽酶-4活性的影响[J].世界中医药,2023,(01):. |
|
| 基于2型糖尿病小鼠模型探索生品黑豆、淡豆豉对二肽基肽酶-4活性的影响 |
| Effects of Black Soybeans and Sojae Semen Praeparatum on Activity of Dipeptidyl Peptidase Ⅳ Based on Mouse Model of Type 2 Diabetes |
| 投稿时间：2021-01-23 |
| DOI：10.3969/j.issn.1673-7202.2023.01.012 |
| 中文关键词: 淡豆豉 生品黑豆 二肽基肽酶-4 胰高血糖素样肽-1 葡萄糖依赖性胰岛素释放肽 2型糖尿病小鼠 血糖 血脂 |
| English Keywords:Sojae Semen Praeparatum Black soybeans Dipeptidyl peptidase-Ⅳ Glucagon-like peptide-1 Glucose-dependent insulinotropic peptide Type 2 diabetic mice Blood sugar Blood fat |
| 基金项目:新疆医科大学附属中医医院院级课题（ZYY2018PZ1）；新疆维吾尔自治区自然科学基金资助项目（2020D01C144） |
|
| 摘要点击次数: 530 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：比较生品黑豆、淡豆豉对2型糖尿病小鼠模型二肽基肽酶-4（DPP-4）活性的影响。方法：建立高糖高脂饮食联合链脲佐菌素（STZ）诱导的2型糖尿病小鼠模型，造模成功后随机分成正常对照组、模型组、阳性对照组、生品黑豆低剂量组、生品黑豆中剂量组、生品黑豆高剂量组、淡豆豉低剂量组、淡豆豉中剂量组、淡豆豉高剂量组。给药期间监测动物血糖变化情况，采用酶法检测血清中总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平，用酶联免疫吸附试验测定血清中DPP-4活性、胰高血糖素样肽-1（GLP-1）、葡萄糖依赖性胰岛素释放肽（GIP）水平。结果：药物干预期间，阳性对照组和淡豆豉高剂量组血糖下降最为显著（P<0.05）。与模型组比较，淡豆豉中剂量组中仅HDL-C水平相对升高，淡豆豉高剂量组中TC、TG、LDL-C、HDL-C水平均有明显变化（P<0.05）；与模型组比较，阳性对照组、生品黑豆高剂量组、淡豆豉中剂量组、淡豆豉高剂量组小鼠血清中DPP-Ⅳ活性抑制率均有升高（P<0.01），阳性对照组和淡豆豉高剂量组GLP-1、GIP水平明显高于模型组（P<0.05）。结论：淡豆豉提取物能够降低2型糖尿病小鼠血糖、血脂等相关生化指标，对2型糖尿病小鼠DPP-4活性有抑制作用，同时可以提高GLP-1、GIP水平。 |
| English Summary: |
| To compare the effects of black soybeans and Sojae Semen Praeparatum(SSP) on the activity of dipeptidyl peptidase Ⅳ(DPP-4) in the mouse model of type 2 diabetes.Methods:The model of type 2 diabetes was induced in mice by high-sugar and high-fat diet combined with streptozotocin(STZ).After modeling，the mice were randomly divided into a normal control group，a model control group，a positive control group，low-，medium-，and high-dose black soybeans groups，and low-，medium-，and high-dose SSP groups.The blood sugar changes of the mice were monitored during drug administration.The serum levels of total cholesterol(TC)，triglyceride(TG)，high-density lipoprotein cholesterol(HDL-C)，and low-density lipoprotein cholesterol(LDL-C) were detected by the enzymatic method.The DPP-4 activity，glucagon-like peptide-1(GLP-1)，and glucose-dependent insulinotropic peptide(GIP) in serum were determined by ELISA.Results:The blood sugar in the positive control group and the high-dose SSP group decreased significantly during drug intervention(P<0.05).Compared with the results in the model control group，only the HDL-C level increased in the medium-dose SSP group，and the levels of TC，TG，LDL-C，and HDL-C in the high-dose SSP group changed significantly(P<0.05).Compared with the model control group，the positive control group，the high-dose black soybeans group，and medium-and high-dose SSP groups showed increased inhibition rates of DPP-Ⅳ activity in serum(P<0.01).The levels of GLP-1 and GIP in the positive control group and the high-dose SSP group were higher than those in the model control group(P<0.05).Conclusion:SSP extract can reduce blood sugar，blood fat，and other biochemical indicators in the mouse model of type 2 diabetes to varying degrees，inhibit DPP-4 activity，and increase the levels of GLP-1 and GIP. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
