| 引用本文:李丹1，胡凯文2，韩丽3，赵百孝4.艾灸对癌性疲劳小鼠AKT-NF-κB信号通路的影响[J].世界中医药,2023,(01):. |
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| 艾灸对癌性疲劳小鼠AKT-NF-κB信号通路的影响 |
| Effect of Moxibustion on AKT-NF-κB Signaling Pathway of Cancer-related Fatigue Mice |
| 投稿时间：2022-01-24 |
| DOI：10.3969/j.issn.1673-7202.2023.01.013 |
| 中文关键词: 艾灸 红外灸 癌性疲劳 炎症介质 AKT-NF-κB信号通路 荷瘤动物 顺铂 |
| English Keywords:Moxibustion Infrared moxibustion Cancer-related fatigue Inflammatory mediator AKT-NF-κB signaling pathway Tumor-bearing animals Cisplatin |
| 基金项目:国家重点研发计划项目（2019YFC1711904） |
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| 中文摘要: |
| 目的：观察艾灸对癌症相关性疲劳（CRF）的作用及对AKT-NF-κB信号通路的影响。方法：选取BALB/C小鼠55只，随机分为空白组（n=10）、对照组（n=15）、艾灸组（n=15）、红外组（n=15）。空白组无任何处理，对照组选择4T1乳腺癌细胞系皮下移植联合顺铂构建CRF动物模型，艾灸组及红外组分别予以艾灸、红外照射CRF小鼠关元穴4周。干预治疗后检测小鼠行为学（疲劳跑台实验、悬尾实验），并运用酶联免疫吸附试验检测血清白细胞介素（IL）含量（IL-1β、IL-6、IL-10），免疫荧光检测脾脏IL-6表达，反转录聚合酶链反应（RT-PCR）检测肿瘤组织IL-6、核因子κB（NF-κB）p65信使核糖核酸(mRNA)表达，蛋白质印迹法（Western Blotting）检测肿瘤组织AKT、p-AKT、NF-κB p65、p-NF-κB p65蛋白表达。结果：与空白组比较，对照组小鼠疲劳跑台时间及距离，IL-10减少，跑台电击次数及悬尾时间，IL-1β、IL-6血清含量，脾脏IL-6荧光表达增加；与对照组比较，干预组小鼠跑台时间及距离，IL-10增加，跑台电击次数和悬尾不动时间，IL-1β、IL-6含量及脾脏IL-6荧光表达，IL-6 mRNA、NF-κB p65mRNA、p-AKT与p-NF-κB p65减少；与红外组比较，艾灸组小鼠悬尾不动时间、血清IL-6、p-NF-κB p65表达减少。结论：艾灸可改善CRF，其机制可能是通过下调AKT-NF-κB信号通路发挥作用，而红外线是艾灸起效的重要因素。 |
| English Summary: |
| To observe the effect of moxibustion on cancer-related fatigue(CRF) and the AKT-NF-κB signaling pathway.Methods:A total of 55 BALB/C mice were randomly divided into blank group(n=10)，control group(n=15)，moxibustion group(n=15) and infrared group(n=15).The blank group received no treatment，while the control group was given subcutaneous transplantation of 4T1 breast cancer cell line combined with cisplatin to construct a CRF model.The moxibustion group and the infrared group were treated with moxibustion and infrared irradiation，respectively at Guanyuan(CV 4) of CRF mice for 4 weeks.After intervention，the behavior of mice(treadmill fatigue test and tail suspension test) was observed，and enzyme linked immunosorbent assay(ELISA) was used to detect the serum level of interleukins(IL-1β，IL-6，and IL-10).Immunofluorescence was used to determine the expression of IL-6 in spleen.Reverse transcription-polymerase chain reaction(RT-PCR) was performed to determine the mRNA expressions of IL-6 and NF-κB p65 in tumor tissues，and the protein expressions of AKT，p-AKT，NF-κB p65 and p-NF-κB p65 in tumor tissues were detected by Western blot.Results:Compared with the blank group，the control group presented shortened treadmill time and distance，decreased IL-10，increased number of electric shocks，prolonged tail suspension time，and elevated serum levels of IL-1β and IL-6 and expression of IL-6 in spleen.Compared with the conditions in the control group，the treadmill time and distance and IL-10 were increased，and the number of electric shocks，tail suspension time，elevated serum levels of IL-1β and IL-6 and up-regulated expression of IL-6 in spleen; the mRNA expressions of IL-6 and NF-κB p65 as well as the expressions of p-AKT and p-NF-κB p65 in the intervention groups were decreased.The infrared group had shorter tail suspension time and lower serum levels of IL-6 and p-NFκB p65 than the moxibustion group.Conclusion:Moxibustion can improve CRF via the mechanism of down-regulating AKT-NF-κB signaling pathway，and infrared is an important factor for the efficacy of moxibustion. |
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