世界中医药

目的：研究灯盏花素对血管内皮细胞损伤大鼠的保护机制及对Wnt/β-catenin信号通路的作用机制。方法：选取60只SD大鼠，随机分为健康组、模型组、灯盏花素低剂量组（BL组）、灯盏花素高剂量组（BH组），每组15只。采用苏木精-伊红（HE）染色观察血管形态；逆转录PCR测Wnt信使RNA（mRNA）、卷曲蛋白1（FZD1） mRNA、轴蛋白1(AXIN1) mRNA、糖原合成酶激酶-3β(GSK-3β) mRNA、β-连环素 mRNA、血管内皮生长因子(VEGF) mRNA；酶联免疫吸附试验测定肿瘤坏死因子-α(TNF-α)、C反应蛋白（CRP）；显微镜观察血管内皮细胞形态；MTT测血管内皮细胞增殖；蛋白质印迹法（Western Blotting）测Wnt、FZD 1、AXIN1、GSK-3β、β-连环素、VEGF水平。结果：健康组大鼠血管内无明显改变；模型组有明显血管损伤；BL组与BH组大鼠血管损伤有所改善，且以BH组效果明显。与健康组比较，模型组血管内皮细胞间隙宽、细胞形态变小、脱落；BL组血管内皮细胞与模型组较为接近；BH组血管内皮细胞间隙变窄且凋亡减少，形态有所改善。与健康组比较，模型组Wnt、VEGF、FZD1、β-连环素、AXIN1、血管内皮细胞不同时间点OD值均降低，GSK-3β、TNF-α、CRP、血管内皮细胞凋亡率升高（P<0.05），与模型组比较，BL组与BH组Wnt、VEGF、FZD1、β-连环素、AXIN1、血管内皮细胞不同时间点OD值均升高，GSK-3β、TNF-α、CRP、血管内皮细胞凋亡率降低（P<0.05），且以BH组效果更为显著（P<0.05）。结论：灯盏花素通过调控Wnt/β-catenin信号通路表达，控制血管的稳定性，从而对血管内皮细胞损伤大鼠的起保护作用。

English Summary:

To study the protective mechanism of breviscapine on rats with vascular endothelial cell injury and its mechanism on the Wnt/β-catenin signaling pathway.Methods:Sixty SD rats were randomly divided into a healthy group，a model group，a low-dose breviscapine group(BL group)，and a high-dose breviscapine group(BH group)，with 15 rats in each group.Blood vessel morphology was observed by HE staining，and the mRNA expression of Wnt，FZD1，AXIN1，GSK-3β，β-catenin，and VEGF was detected by RT-PCR.TNF-α and CRP were measured by ELISA.The morphology of vascular endothelial cells was observed under the microscope.MTT was used to measure vascular endothelial cell proliferation.The levels of Wnt，FZD1，AXIN1，GSK-3β，β-catenin，and VEGF were measured by Western blot.Results:There were no obvious changes in the blood vessels of healthy rats.The model rats showed obvious vascular injury.Vascular injury was improved in the BL group and the BH group，especially in the BH group.Compared with the healthy group，the model group showed wider space between vascular endothelial cells and smaller and exfoliated cells.The vascular endothelial cells of the BL group were similar to those of the model group.The BH group had narrowed vascular endothelial cell space and reduced apoptosis，and the morphology was improved.Compared with the healthy group，the model group showed decreased OD values of Wnt，VEGF，FZD1，β-catenin，AXIN1，and vascular endothelial cells at different time points，and increased apoptosis rates of GSK-3β，TNF-α，CRP，and vascular endothelial cells(P<0.05).Compared with the model group，the BL and BH groups showed increased OD values of Wnt，VEGF，FZD1，β-catenin，AXIN1，and vascular endothelial cells at different time points and decreased apoptosis rates of GSK-3β，TNF-α，CRP，and vascular endothelial cells(P<0.05)，and such changes were more significant in the BH group(P<0.05).Conclusion:Breviscapine can control vascular stability by regulating the expression of the Wnt/β-catenin pathway，thereby protecting against vascular endothelial cell injury in rats.

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