To explore the potential mechanism of Yiyi Fuzi Baijiang San in the treatment of pelvic inflammatory diseases(PID) by network pharmacology，and to verify the related inflammatory indexes by animal experiments.Methods:The components and targets of Yiyi Fuzi Baijiang San were searched by “Traditional Chinese Medicine System Pharmacology Database and Analysis platform(TCMSP)”.The target genes of PID were searched by Genecard and OMIM database.The “disease-drug” protein-protein interaction network map was drawn by visualization tools and enriched and analyzed by Gene Ontology(GO) functional enrichment analysis and Kyoto Encyclopedia of Gene and Genome(KEGG) pathway enrichment analysis.The relevant inflammatory indexes were verified by animal experiments.Results:There were 21 active components and 197 target genes in the treatment of PID with Yiyi Fuzi Baijiang San and 47 overlapping genes in the “disease-drug” network.There were 77 items in GO enrichment，which were mainly related to oxidative stress and epithelial cell proliferation.KEGG enrichment analysis had 125 pathways，including advanced glycation end product receptor-ligand pathway，liquid shear stress and atherosclerosis，mitogen-activated protein kinase signal pathway，and so on.These pathways were associated with inflammation.Therefore，it was speculated that Yiyi Fuzi Baijiang San may treat PID with anti-inflammation.Animal experiments showed that Yiyi Fuzi Baijiang San decreased the levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in serum and uterine tissues of rats with PID and increased the level and expression of interleukin-10(IL-10) in a dose-dependent manner.Conclusion:Yiyi Fuzi Baijiang San can treat PID by regulating the levels of IL-6，IL-10，TNF-α，and protein expression.