| 引用本文:惠鑫1，2，王昊1，3，郭洪祝4，林珉瑜5，欧阳夏荔1，吴嘉威1，于密密4，赵百孝6.艾绒混伪品鉴别方法[J].世界中医药,2023,(02):. |
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| 艾绒混伪品鉴别方法 |
| Identification of Moxa Floss Adulterant |
| 投稿时间：2021-12-27 |
| DOI：10.3969/j.issn.1673-7202.2023.02.005 |
| 中文关键词: 艾绒 薄层色谱 气相色谱-质谱联用 艾绒鉴别 艾灸 艾绒标准化 艾叶 |
| English Keywords:Moxa floss TCL GC-MS Identification of moxa floss Moxibustion Standardization of moxa floss Folium Artemisiae Argyi |
| 基金项目:国家重点研发计划“中医药现代化研究”重点专项（2019YFC1711900）；国家标准委国家标准项目（20170320-T-468）；国家自然科学基金——外国青年学者研究基金项目（81850410548） |
| 作者 | 单位 | | 惠鑫1，2，王昊1，3，郭洪祝4，林珉瑜5，欧阳夏荔1，吴嘉威1，于密密4，赵百孝6 | 1 北京中医药大学针灸推拿学院,北京,100029； 2 北京航天总医院,北京,100076； 3 解放军总医院第六医学中心,北京,100037； 4 北京市药品检验研究院,北京,102206； 5 北京中医药大学国际学院,北京,100029； 6 北京中医药大学东直门医院,北京,100007 |
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| 中文摘要: |
| 目的：通过对艾绒的薄层色谱鉴别,气相色谱-质谱联用（GC-MS）成分分析,初步建立艾绒混伪品的鉴别方法。方法：将正品艾绒、混伪品艾绒加石油醚（60~90 ℃）加热回流,蒸干后加环己烷溶解作为供试品溶液,另取艾叶对照药材同法制备对照药材溶液。吸取供试品溶液和对照溶液各2 μL分别点于同一硅胶G薄层板和反向薄层色谱硅胶预制板,于日光下及紫外光灯（365 nm）下检视。采用GC-MS测定艾绒挥发油类成分比较正品艾绒与艾绒混伪品的气相色谱图差异。结果：硅胶薄层色谱及C18反相薄层色谱均可在日光、紫外光灯（365 nm）下出现特异性斑点。气相色谱图显示混伪品艾绒在挥发油类成分含量上存在差异,且检出了正品艾绒中没有检出的成分（十五烷、菊薁）。结论：采用硅胶薄层色谱、C18反相薄层色谱2种方法能较快、较准确地鉴别艾绒混伪品,结果稳定。GC-MS能够检测出混伪品艾绒的组分差异。 |
| English Summary: |
| Thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) were used to identify moxa floss adulterant.Methods:The authentic moxa floss and adulterant were heated under reflux with petroleum ether (60~90 ℃)，evaporated to dryness，and then dissolved in cyclohexane to yield the test solution.The reference Folium Artemisiae Argyi was used to prepare the reference solution with the same method.A total of 2 μL of test solution and 2 μL of reference solution were respectively dripped on the same Merck TLC silica gel plate and reversed-phase TLC silica gel precoated plate and observed under sunlight and ultraviolet light (365 nm).GC-MS was used to determine the volatile oil constituents of moxa floss and gas chromatograms of the authentic product and the adulterant were compared.Results:Silica gel TLC plate and C18 reversed-phase TLC plate showed specific spots under sunlight and ultraviolet light (365 nm).According to the gas chromatograms，the content of volatile oil constituents was different between the authentic moxa floss and the adulterant and pentadecane and chamazulen which were found in the adulterant failed to be detected in authentic moxa floss.Conclusion:Silica gel TLC and C18 reversed-phase TLC can quickly and accurately distinguish moxa floss adulterant，with stable result.GC-MS can be used to detect the differential components in the adulterant. |
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