| 引用本文:高艳艳，陈晓鹤，苏磊，张薇，蒋丽娟，姜艳艳，刘斌.高效液相色谱法指纹图谱结合化学模式识别的地骨皮饮片质量研究[J].世界中医药,2023,(05):. |
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| 高效液相色谱法指纹图谱结合化学模式识别的地骨皮饮片质量研究 |
| Quality of Lycii Cortex Pieces Based on HPLC Fingerprint and Chemical Pattern Recognition |
| 投稿时间：2021-11-18 |
| DOI：10.3969/j.issn.1673-7202.2023.05.001 |
| 中文关键词: 地骨皮 指纹图谱 化学模式识别 咖啡酰丁二胺 地骨皮乙素 绿原酸 |
| English Keywords:Lycii Cortex Fingerprint Chemical pattern recognition N-caffeoylputrescine Kukoamine B Chlorogenic acid |
| 基金项目:北京中医药大学“青年科学家培育计划”科研新锐人才项目（BUCM-2019-QNKXJC008） |
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| 中文摘要: |
| 目的：建立地骨皮饮片的高效液相色谱法（HPLC）指纹图谱，结合化学模式识别技术评价地骨皮饮片的质量。方法：采用HPLC建立10批地骨皮饮片的指纹图谱，利用中药色谱指纹图谱相似度评价系统2004A进行相似度评价，确定共有峰；以生成的对照指纹图谱检验45批市售不同产区地骨皮饮片的质量；采用聚类分析、主成分分析评价地骨皮饮片质量。结果：建立了地骨皮饮片HPLC指纹图谱，共标定14个共有峰，相似度均在0.90以上；45批地骨皮饮片的相似度为0.282~0.998，整体质量存在一定差别；聚类分析将45批地骨皮饮片聚为5类；主成分分析显示45批地骨皮饮片综合得分以D19、D18、D22较高。结论：指纹图谱结合化学模式识别可实现对地骨皮饮片的科学、准确评价，为地骨皮饮片的质量评价和合理应用提供了科学依据。 |
| English Summary: |
| To evaluate the quality of Lycii Cortex pieces by establishing HPLC fingerprint combined with chemical pattern recognition.Methods:HPLC fingerprints of 10 batches of Lycii Cortex pieces were established.Similarity evaluation was performed by using Similarity Evaluation System for Chromatographic Fingerprint of TCM(2004A) to confirm the common peaks.The quality of 45 batches of Lycii Cortex pieces from different regions was evaluated by the reference fingerprint.Principal component analysis(PCA) and hierarchical cluster analysis(HCA) were used for the comprehensive quality evaluation of Lycii Cortex pieces.Results:The HPLC fingerprints of Lycii Cortex pieces were established，and 14 common peaks were marked，with the similarity above 0.90.The similarity of 45 batches of samples was between 0.282 and 0.998，and there were some differences in overall quality.Forty-five batches of Lycii Cortex pieces were clustered into five categories.The results of PCA showed that D19，D18，and D22 got the highest comprehensive scores.Conclusion:HPLC fingerprint combined with chemical pattern recognition can realize the rapid and accurate evaluation of Lycii Cortex pieces，which provides a reference for the quality evaluation and rational usage of Lycii Cortex pieces. |
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