| 引用本文:陈健华1，周小毛1，黄卓1，余尚贞2.冰黄五苓散对大脑中动脉闭塞模型大鼠的干预作用[J].世界中医药,2023,(07):. |
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| 冰黄五苓散对大脑中动脉闭塞模型大鼠的干预作用 |
| Effect of Binghuang Wuling Powder in the Treatment of Middle Cerebral Artery Occlusion in Rats |
| 投稿时间：2021-06-19 |
| DOI：10.3969/j.issn.1673-7202.2023.07.015 |
| 中文关键词: 缺血性中风 脑水肿 冰黄五苓散 水孔蛋白4 神经元特异性烯醇化酶 S100β蛋白 |
| English Keywords:Ischemic stroke Cerebral edema Binghuang Wuling Powder Aquaporin 4 Neuron-specific enolase S100β |
| 基金项目:广东省中医药管理局科研项目（20183016） |
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| 中文摘要: |
| 目的：观察冰黄五苓散直肠滴注对于大脑中动脉闭塞（MCAO）模型大鼠的血清神经元特异性烯醇化酶(NSE)、S100β蛋白、脑组织水孔蛋白4(AQP4)的影响。方法：将90只SD大鼠随机分为假手术组、模型组、冰黄五苓散组。对各组大鼠不同时间点的神经功能缺损进行评分，采用干-湿重法测定脑组织含水量，采用酶联免疫吸附试验（ELISA）检测血清NSE、S100β蛋白水平，采用免疫组织化学法检测脑组织AQP4表达。结果：冰黄五苓散组神经功能缺损评分、脑组织含水量明显低于模型组（P<0.05）;冰黄五苓散组血清NSE、S100β蛋白、脑组织AQP4明显低于模型组（P<0.05）。结论：冰黄五苓散能够改善MCAO模型大鼠脑水肿情况，起到保护神经功能的作用。 |
| English Summary: |
| To observe the effect of rectoclysis with Binghuang Wuling Powder on neuron-specific enolase(NSE)，S100β，and aquaporin 4(AQP4) in rats with middle cerebral artery occlusion(MCAO).Methods:Ninety SD rats were randomly assigned into a sham operation group，a model group，and a Binghuang Wuling Powder group.The rats in each group were scored for neurological deficits at different time points.The brain water content was measured by dry-wet weight method.The serum levels of NSE and S100β were determined by enzyme-linked immunosorbent assay(ELISA)，and AQP4 expression in the brain tissue by immunohistochemistry.Results:The Binghuang Wuling Powder group had lower neurological deficit score，brain water content，serum NSE and S100β levels，and AQP4 expression level than the model group(P<0.05).Conclusion:Binghuang Wuling Powder can mitigate cerebral edema and protect nerve function in the treatment of MCAO rats. |
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