世界中医药

基金项目:国家自然科学基金项目（82074181）；福建省科技计划高校联合项目（2019J01352）；福建省康复重点实验室联合福建省康复产业研究院开放课题（2015Y2001，KF2019004）；福建省教育厅中青年教师项目（JAT190233，JAT190232）；福建中医药大学校管课题（X2019022，X2018016）；福建中医药大学校管课题（X2019010-人才专项）

作者	单位
黄于婷1，杨岚菲1，屈伸华1，陈佳彦1，方燕平1，刘阅阅1，郑林遥1，王笛钇1，康小玲1，童秀冰1，阚宇1，2，廖军1，2，景向红1，3	1 福建中医药大学针灸学院，福州，350122； 2 福建省康复技术重点实验室，福州，350122； 3 中国中医科学院针灸研究所，北京，100700

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中文摘要:

目的：观察电针对大鼠颈肌慢性损伤模型肌电活动、颈后肌细胞凋亡的影响。方法：34只Wistar大鼠选取7只为空白对照组，其余大鼠复制颈肌慢性损伤模型备用。（经超声诊断仪检测，6只大鼠模型复制不成功，剔除。）将模型大鼠随机分为模型组、电针组、美洛昔康组，每组7只。电针组针刺双侧“风池穴”，1次/d，25 min/次，连续治疗10 d为1个疗程，疗程之间间隔2 d，共2个疗程。使用超声诊断仪检测大鼠颈后肌组织的形态学变化，电生理记录大鼠颈后肌肌电变化，脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL法）检测细胞凋亡率，免疫组织化学法标记B细胞淋巴瘤-2相关X蛋白（Bax）、B淋巴细胞瘤-2（Bcl-2）、胱天蛋白酶-3（Caspase-3）、Caspase-9蛋白的表达情况。结果：超声检测结果显示：与模型组比较，电针后肌纤维排列较整齐，肌厚度增粗。肌电生理检测结果显示：模型组肌电波幅及频率明显发生了衰减，电针后肌电波幅和频率明显高于模型组。TUNEL结果显示：与模型组比较，电针组凋亡细胞数目明显减少，差异有统计学意义（均P<0.05）。免疫组织化学结果显示：与空白对照组比较，模型组Bcl-2的阳性细胞数少于空白组，Bax、Caspase-3、Caspase-9的阳性细胞数增多（P<0.05）；与模型组比较，电针组中的Bcl-2的阳性细胞数增多，Bax、Caspase-3、Caspase-9的阳性细胞数减少（P<0.05）。结论：电针干预可修复颈肌慢性损伤，其机制可能与提高慢性颈肌损伤大鼠颈肌肌电振幅及抑制细胞凋亡相关，其途径可能通过抑制线粒体通路的活性而实现。

English Summary:

To observe the effects of electropuncture(EA) on myoelectric activity and apoptosis of posterior cervical muscle cells in rats with chronic cervical muscle injury.Methods:Seven Wistar rats were randomly selected from 34 Wistar rats and assigned to the blank group，and the remaining rats were used to establish a chronic cervical muscle injury model(as detected by ultrasonic diagnosis，six rats failed for model induction and were excluded).The model rats were randomly divided into a model group，an EA group，and a meloxicam group，with seven rats in each group.The rats in the EA group received EA treatment at bilateral Fengchi(GB 20)，25 min each time，once a day，for 10 consecutive days as a course of treatment，with a two-day interval between the two courses of treatment，for a total of two courses.An ultrasonic diagnostic system was used to detect the morphological changes in rat posterior cervical muscle tissues，and electrophysiological records presented the changes in rat cervical muscle myoelectricity.Cell apoptosis rate was detected using the TUNEL method，and protein expression levels of B-cell lymphoma 2(Bcl-2)，Bcl-2-associated X protein(Bax)，cysteinyl aspartate-specific protease 3(Caspase-3)，and cysteinyl aspartate-specific protease 9(Caspase-9) were detected by immunohistochemistry.Results:The results of the ultrasonic examination showed that the arrangement of muscle fibers was orderly and the muscle thickness increased after EA treatment as compared with the conditions in the model group.The results of the electrophysiological test showed that the amplitude and frequency of myoelectricity in the model group were significantly attenuated，and the amplitude and frequency of myoelectricity after EA were significantly higher than those in the model group.TUNEL results showed that the number of apoptotic cells in the EA group was significantly reduced compared with that in the model group(P<0.05).Immunohistochemical results showed that the number of Bcl-2 positive cells in the model group was lower and the number of Bax，Caspase-3，and Caspase-9 positive cells was higher than those in the blank group(P<0.05).The number of Bcl-2 positive cells in the EA group was higher，and the number of Bax，Caspase-3，and Caspase-9 positive cells was lower than those in the model group(P<0.05).Conclusion:EA can repair chronic cervical muscle injury，and its mechanism may be attributed to increasing the amplitude of cervical muscle electromyography and inhibiting apoptosis in rats with chronic cervical muscle injury，which may be realized by inhibiting the activity of the mitochondrial pathway.

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