| 引用本文:刘丽娟1，赵亚1，吴亚运1，赵瑞芝1，2.醋柴胡多糖对拉米夫定体外抗乙型肝炎病毒的增效作用研究[J].世界中医药,2023,(08):. |
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| 醋柴胡多糖对拉米夫定体外抗乙型肝炎病毒的增效作用研究 |
| Synergistic Effect of Polysaccharide from Vinegar-baked Radix Bupleuri with Lamivudine Against Hepatitis B Virus in Vitro |
| 投稿时间：2021-09-27 |
| DOI：10.3969/j.issn.1673-7202.2023.08.004 |
| 中文关键词: 醋柴胡多糖 乙型肝炎病毒 乙型肝炎e抗原 乙肝病毒脱氧核糖核酸 协同增效 拉米夫定 有机阳离子转运蛋白1 P糖蛋白 多药耐药蛋白2 |
| English Keywords:Polysaccharide from vinegar-baked radix bupleuri(VBCP) Hepatitis B virus(HBV) Hepatitis B e antigen(HBeAg) HBV-DNA Synergistic effect lamivudine(LAM) Organic cation transporter 1(OCT1) P-glycoprotein(P-gp) Multidrug resistance protein 2(MRP2) |
| 基金项目:国家自然科学基金面上项目（82173981）；广东省科技计划项目（2017A020211013;2017B030314166） |
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| 中文摘要: |
| 目的：考察醋柴胡多糖对拉米夫定抗乙型肝炎病毒（HBV）的增效作用，并初步探讨其作用机制。方法：将不同浓度的醋柴胡多糖、拉米夫定及二者联合作用于人肝癌细胞HepG2.2.15，同时设立对照组，孵育48 h，酶联免疫吸附试验试剂盒检测细胞上清液中乙型肝炎表面抗原（HBsAg)、乙型肝炎e抗原(HBeAg)分泌量，荧光探针定量聚合酶链式反应（PCR）检测细胞HBV脱氧核糖核酸（DNA）表达量，金（正均）氏公式定量分析醋柴胡多糖的增效作用。采用高效液相色谱法测定细胞内拉米夫定含量；Western blot法测定有机阳离子转运蛋白（OCT）1、OCT2、P糖蛋白（P-gp）和多药耐药蛋白2（MRP2)的表达量。结果：与拉米夫定单用组相比，醋柴胡多糖增加拉米夫定对HBsAg分泌的抑制作用，表现为相加作用、对HBeAg作用表现为协同增强，Q值达6.55、对HBV-DNA抑制作用表现为相加。醋柴胡多糖低剂量组、醋柴胡多糖低中剂量组、醋柴胡多糖低高剂量组可显著促进拉米夫定的摄取；醋柴胡多糖高剂量联用组可显著降低P-gp的表达；醋柴胡多糖单用及联用组均可显著提高OCT1的表达。结论：醋柴胡多糖可通过增加拉米夫定的摄取发挥协同抗HBV作用，其作用机制可能P-gp、OCT1有关。 |
| English Summary: |
| To study the synergistic effect of polysaccharide from vinegar-baked radix bupleuri(VBCP) with lamivudine(LAM) against hepatitis B virus(HBV) and investigate the mechanism of action.Methods:VBCP，LAM，and VBCP combined with LAM of different concentrations were added into HepG2.2.15 cells.At the same time，the control group was set up and incubated for 48 h.The secretion levels of hepatitis B surface antigen(HBsAg) and hepatitis B e antigen(HBeAg) in the supernatant of the cells were detected by enzyme-linked immunosorbent assay kit，and fluorescence probe quantitative polymerase chain reaction(PCR) was used to detect HBV-deoxyribonucleic acid(DNA) expression in cells.Quantitative analysis of the synergistic effect of polysaccharide from VBCP was conducted by Jin(Zhengjun) formula.The intracellular LAM content was determined by high-performance liquid chromatography.The expression levels of organic cation transporter(OCT) 1，OCT2，P-glycoprotein(P-gp)，and multidrug resistance protein 2(MRP2) were determined by Western blot.Results:Compared with mono-LAM treatment，polysaccharide from VBCP increased the inhibitory effect of LAM on HBsAg secretion，showing an additive effect，and the effect on HBeAg showed a synergistic enhancement.Q value was 6.55，and the inhibitory effect on HBV-DNA was additive.Low-dose，low and middle-dose，and low and high-dose groups of polysaccharide from VBCP could significantly promote the uptake of LAM.The expression of P-gp was significantly decreased in the combined group with a high dose of polysaccharide from VBCP，and groups with polysaccharide from VBCP alone and VBCP combined with LAM significantly increased the expression of OCT1.Conclusion:Polysaccharide from VBCP can play a synergistic anti-HBV effect by increasing the uptake of LAM，and its mechanism may be related to P-gp and OCT1. |
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