To analyze the effects of Danggui Shaoyao Powder and its modified prescriptions on the levels of blood lipid，interleukin-6(IL-6)，interleukin-8(IL-8)，and c-Jun N-terminal kinase(JNK) in high-fat diet-induced atherosclerosis(AS) ApoE-/-mice.Methods:Seventy-two ApoE-/-mice were randomly divided into a model group，a Danggui Shaoyao Powder group，a Jianpi Huazhuo group，an atorvastatin calcium group，a Huoxue Sanyu group，and a Rougan Huanji group，with 12 mice in each group.The AS model was established by the high-fat diet.Twelve C57BL/6J mice were fed with a normal diet and were assigned to the blank group.After one week of adaptive feeding，mice in each group with drug intervention received Chinese medicines by gavage for 16 weeks.An automatic biochemical analyzer was adopted to detect serum total cholesterol(TC)，triacylglycerol(TG)，high-density lipoprotein cholesterol(HDL-C)，and low-density lipoprotein cholesterol(LDL-C) levels.Enzyme-linked immunosorbent assay(ELISA) was used to detect the expression levels of IL-6，IL-8，and JNK in mouse aorta.Results:Compared with the normal group，the model group showed increased TC，TG，and LDL-C，and decreased HDL-C(P<0.01).Compared with the model group，the Danggui Shaoyao Powder group，the Jianpi Huazhuo group，the atorvastatin calcium group，the Huoxue Sanyu group，and the Rougan Huanji group showed decreased levels of TC，TG，and LDL-C and increased HDL-C in AS ApoE-/-mice(P<0.01).Compared with the normal group，the model group showed up-regulated expression of IL-6，IL-8，and JNK(P<0.01).Compared with the model group，the groups with drug intervention showed down-regulated IL-6，IL-8，and JNK expression(P<0.01).Conclusion:Danggui Shaoyao Powder and its modified prescriptions can significantly improve the levels of blood lipids and reduce the levels of inflammation-related factors in mice，thus alleviating the development of AS.Among them，the effect of Jianpi Huazhuo group is more obvious than that of other groups.