世界中医药
文章摘要
引用本文:宗毓麟,孙月蒙,孙燕,周璐,鹿艺星,李昱达,张淑静,高誉珊,郑丰杰,李宇航.当归芍药散及其拆方对动脉粥样硬化小鼠血脂及炎症介质的影响[J].世界中医药,2023,(09):.  
当归芍药散及其拆方对动脉粥样硬化小鼠血脂及炎症介质的影响
Effects of Danggui Shaoyao Powder and its Modified Prescriptions on Blood Lipid and Inflammatory Factors in Atherosclerotic Mice
投稿时间:2022-09-01  
DOI:10.3969/j.issn.1673-7202.2023.09.003
中文关键词:  动脉粥样硬化  载脂蛋白E敲除小鼠  当归芍药散  血脂  炎症介质  方剂要素  健脾化浊
English Keywords:Atherosclerosis  ApoE-/-mice  Danggui Shaoyao Powder  Blood lipid  Inflammatory factor  Prescription Elements  Jianpi Huazhuo
基金项目:国家自然科学基金项目(82074325)
作者单位
宗毓麟,孙月蒙,孙燕,周璐,鹿艺星,李昱达,张淑静,高誉珊,郑丰杰,李宇航 北京中医药大学中医学院北京100029 
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中文摘要:
      目的:探讨当归芍药散及其拆方对高脂饮食诱导的载脂蛋白E敲除(ApoE-/-)小鼠动脉粥样硬化(AS)模型血脂及白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、c-Jun氨基端激酶(JNK)水平的影响。方法:72只ApoE-/-小鼠随机分为模型组、当归芍药散组、健脾化浊组、阿托伐他汀钙组、活血散瘀组和柔肝缓急组,每组12只,高脂饮食诱导建立AS模型。12只C57BL/6J小鼠作为正常组普通饮食饲养。适应性饲养1周后各给药组小鼠给予中药灌胃16周。全自动生化分析仪检测血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)水平;酶联免疫吸附试验法测定小鼠主动脉IL-6、IL-8、JNK表达水平。结果:与正常组比较,模型组小鼠血清TC、TG、LDL-C水平均显著升高(均P<0.01)、HDL-C水平显著降低(P<0.01),差异有统计学意义;与模型组比较,当归芍药散组、健脾化浊组、阿托伐他汀钙组、活血散瘀组、柔肝缓急组均可显著降低ApoE-/-小鼠AS模型TC、TG、LDL-C水平、升高HDL-C水平,差异有统计学意义(P<0.01);与正常组比较,模型组小鼠主动脉IL-6、IL-8和JNK表达显著升高,差异有统计学意义(P<0.01);与模型组比较,各给药组均可显著下调小鼠主动脉IL-6、IL-8、JNK表达水平(P<0.01)。结论:当归芍药散及其拆方可以显著改善小鼠血脂水平,降低炎症介质水平,从而缓解AS的发展。其中健脾化浊组方剂要素较其他拆方组效果更为明显。
English Summary:
      To analyze the effects of Danggui Shaoyao Powder and its modified prescriptions on the levels of blood lipid,interleukin-6(IL-6),interleukin-8(IL-8),and c-Jun N-terminal kinase(JNK) in high-fat diet-induced atherosclerosis(AS) ApoE-/-mice.Methods:Seventy-two ApoE-/-mice were randomly divided into a model group,a Danggui Shaoyao Powder group,a Jianpi Huazhuo group,an atorvastatin calcium group,a Huoxue Sanyu group,and a Rougan Huanji group,with 12 mice in each group.The AS model was established by the high-fat diet.Twelve C57BL/6J mice were fed with a normal diet and were assigned to the blank group.After one week of adaptive feeding,mice in each group with drug intervention received Chinese medicines by gavage for 16 weeks.An automatic biochemical analyzer was adopted to detect serum total cholesterol(TC),triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C) levels.Enzyme-linked immunosorbent assay(ELISA) was used to detect the expression levels of IL-6,IL-8,and JNK in mouse aorta.Results:Compared with the normal group,the model group showed increased TC,TG,and LDL-C,and decreased HDL-C(P<0.01).Compared with the model group,the Danggui Shaoyao Powder group,the Jianpi Huazhuo group,the atorvastatin calcium group,the Huoxue Sanyu group,and the Rougan Huanji group showed decreased levels of TC,TG,and LDL-C and increased HDL-C in AS ApoE-/-mice(P<0.01).Compared with the normal group,the model group showed up-regulated expression of IL-6,IL-8,and JNK(P<0.01).Compared with the model group,the groups with drug intervention showed down-regulated IL-6,IL-8,and JNK expression(P<0.01).Conclusion:Danggui Shaoyao Powder and its modified prescriptions can significantly improve the levels of blood lipids and reduce the levels of inflammation-related factors in mice,thus alleviating the development of AS.Among them,the effect of Jianpi Huazhuo group is more obvious than that of other groups.
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