To study the mechanism of purified protein in Pheretima aspergillum in promoting wound repair.Methods:The Cell Counting Kit-8(CCK-8) assay was used to detect the effect of P.aspergillum purified protein on the proliferation of immortalized human keratinocytes(HaCaT cells) and mouse fibroblasts(NIH3T3 cells).The cell scratch test was used to investigate the effect on cell migration of HaCaT and NIH3T3 cells.The hydroxyproline assay kit and the enzyme-linked immunosorbent assay(ELISA) were used to detect the levels of hydroxyproline(HYP)，collagen-Ⅰ(Col-Ⅰ)，and matrix metalloproteinase-1(MMP-1) in the culture supernatant of NIH3T3 cells.Results:Within a certain concentration range，P.aspergillum purified protein could promote the proliferation and migration of HaCaT and NIH3T3 cells，with the strongest cell proliferation activity and the highest scratch healing rate at 0.313 mg/mL and 1.250 mg/mL，respectively.After administration of P.aspergillum purified protein，the levels of HYP and Col-Ⅰ in NIH3T3 cell culture supernatant increased significantly，while the level of MMP-1 decreased significantly.The administration of P.aspergillum purified protein at the concentration of 1.250 mg/mL showed the most significant results compared with the control group.Conclusion:P.aspergillum purified protein can promote the proliferation and migration of HaCaT and NIH3T3 cells，improve the synthesis and secretion of collagen in NIH3T3 cells，and inhibit the degradation of collagen，thereby promoting wound healing.