| 引用本文:陶思冥1，罗建江1，李争1，荆晶1，孟驰2，张艳丽1，李风森1.益气固表丸含药血清对炎症肺上皮细胞TGF-β2-Smad信号通路的影响[J].世界中医药,2023,(09):. |
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| 益气固表丸含药血清对炎症肺上皮细胞TGF-β2-Smad信号通路的影响 |
| Effects of Serum Containing Yiqi Gubiao Pills on TGF-β2-Smad Signaling Pathway in Inflammatory Lung Epithelial Cells |
| 投稿时间：2021-10-29 |
| DOI：10.3969/j.issn.1673-7202.2023.09.005 |
| 中文关键词: 慢性阻塞性肺疾病 益气固表丸 TGF-β2-Smad信号通路 炎症介质 含药血清 |
| English Keywords:Chronic obstructive pulmonary disease Yiqi Gubiao Pills TGF-β2-Smad signaling pathway Inflammatory mediator Medicated serum |
| 基金项目:国家自然科学基金项目（81660740）；新疆维吾尔自治区自然科学基金青年项目（2019D01C177） |
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| 中文摘要: |
| 目的：通过采用益气固表含药血清对慢性阻塞性肺疾病(COPD)炎症细胞模型进行干预，以转化生长因子-β2（TGF-β2）-Sma和Mad相关（Smad）信号通路及相关炎症介质为切入点，揭示益气固表丸调节体内炎症水平的可能作用机制。方法：以卷烟烟雾提取物（CSE）和脂多糖（LPS）为刺激因子，以肺上皮细胞A549为载体，建立COPD炎症细胞模型。用益气固表丸对大鼠进行灌胃，2次/d，间隔12 h，给药7 d后采血，制备含药血清。用益气固表丸高、中、低剂量含药血清进行培养干预，运用实时荧光定量PCR法（RT-qPCR）和酶联免疫吸附试验(ELISA)、免疫荧光单标等检测干预前后CD4、CD8、白细胞介素-6（IL-6）、IL-17、肿瘤坏死因子-α(TNF-α）等炎症介质和TGF-β2-Smad信号通路重要分子变化。结果：各给药组CD4显著高于对照组，差异有统计学意义（P<0.05）；各给药组CD8、IL-6、IL-17、TNF-α、TGF-β2、Smad2、Smad3表达水平低于对照组，差异有统计学意义（P<0.05）。结论：益气固表丸含药血清对炎症肺上皮细胞的抑制作用可能与调控TGF-β2-Smad信号通路有关。 |
| English Summary: |
| To reveal the underlying mechanism of Yiqi Gubiao Pills(YQGB) in regulating inflammation in vivo by using YQGB-medicated serum to intervene in the inflammatory cell model of chronic obstructive pulmonary disease(COPD) with the TGF-β2-Smad signaling pathway and related inflammatory factors as the entry point.Methods:The inflammatory cell model of COPD was established with cigarette smoke extract(CSE) and lipopolysaccharide(LPS) as stimulants and lung epithelial A549 cells as the carrier.Rats received YQGB by gavage，twice a day at an interval of 12 h.After 7 days of feeding，blood was collected to prepare drug-containing serum.High-dose，medium-dose，and low-dose YQGB-medicated serum was used for culture intervention.RT-qPCR，ELISA，and immunofluorescence were used to detect the changes of inflammatory cytokines(such as CD4，CD8，IL-6，IL-17，and TNF-α) and important molecules in TGF-β2-Smad signal transduction pathway before and after intervention.Results:CD4 in the groups with drug intervention was significantly higher than that in the control group(P<0.05).The expression levels of CD8，IL-6，IL-17，TNF-α，TGF-β2，Smad2，and Smad3 in the groups with drug intervention were lower than those in the control group(P<0.05).Conclusion:The anti-inflammatory effect of YQGB-medicated serum on inflammatory lung epithelial cells may be related to the regulation of the TGF-β2-Smad signaling pathway. |
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