| 引用本文:钮妍1，国生2，魏培栋2，张茹2，杨靖颐2，刘杨2，王泽中2，宋红志2，付国兵2，王康2.推拿改善运动性疲劳大鼠免疫炎性损伤的作用机制研究[J].世界中医药,2023,(10):. |
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| 推拿改善运动性疲劳大鼠免疫炎性损伤的作用机制研究 |
| Mechanism of Tuina in Improving the Immunoinflammatory Injury of Exercise-induced Fatigue Rats |
| 投稿时间：2021-04-14 |
| DOI：10.3969/j.issn.1673-7202.2023.10.005 |
| 中文关键词: 推拿 运动性疲劳 外周血单个核细胞 炎症介质 氧化应激 运动性免疫抑制 |
| English Keywords:Tuina Exercise-induced fatigue Peripheral blood mononuclear cells Inflammatory factors Oxidative stress Exercise-induced immunosuppression |
| 基金项目:国家自然科学基金面上项目（81574092） |
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| 中文摘要: |
| 目的：观察推拿对运动性疲劳（EF）大鼠外周血炎症介质和外周血单个核细胞（PBMCs）凋亡与亚型平衡的影响，探索推拿改善运动性免疫炎性损伤的机制。方法：选取Wistar大鼠30只，随机将6只作为空白组，其余运用跑台运动法进行造模，将16只成模大鼠随机分为模型组及推拿组，每组8只。空白组不进行任何处理，模型组每日力竭运动后于笼中休息，推拿组每日力竭运动后予以推拿干预1次，共干预21 d。取材后，运用酶联免疫吸附试验（ELISA）法测定外周血白细胞介素-1β（IL-1β）与肿瘤坏死因子-α（TNF-α）含量，流式细胞术测定PBMCs凋亡情况及Th1/Th2，Th17/Treg亚型分布情况。结果：与空白组比较，模型组大鼠外周血中IL-1β和TNF-α的含量显著升高（均P<0.01）；与模型组比较，推拿组外周血中IL-1β和TNF-α含量显著下调（均P<0.01）。与空白组比较，模型组大鼠PBMCs凋亡率显著上升（P<0.01）；与模型组比较，推拿组大鼠PBMCs凋亡率显著下降（P<0.05）。与空白组比较，模型组大鼠PBMCs中Th1和Th17细胞增多，Th2和调节性T（Treg）细胞减少，出现Th1和Th17漂移，Th1/Th2及Th17/Treg细胞比例均显著上调（均P<0.01）；与模型组比较，推拿组大鼠PBMCs中Th1/Th2及Th17/Treg细胞比例均显著下调（P<0.01，P<0.05）。结论：推拿改善了EF大鼠外周血的免疫炎性状态，体现出抗炎、抗凋亡及免疫调节的手法效应。 |
| English Summary: |
| To observe the effects of Tuina on peripheral blood inflammatory mediators and peripheral blood mononuclear cell(PBMC) apoptosis and subtype balance in exercise-induced fatigue(EF) rats and explore the mechanism of Tuina in improving exercise-induced immune-inflammatory injury.Methods:Thirty Wistar rats were selected and six rats were assigned to the blank group.The remaining rats underwent model induction using a treadmill exercise method.Sixteen model rats were divided into a model group(n=8) and a Tuina group(n=8).The blank group did not undergo any treatment.The model group rested in the cage after exhaustive exercise every day，and the Tuina group received Tuina intervention once a day after exhaustive exercise for a total of 21 days.Enzyme-linked immunosorbent assay(ELISA) was used to measure the content of peripheral blood interleukin(IL)-1β and tumor necrosis factor(TNF)-α，while flow cytometry was used to measure PBMC apoptosis and Th1/Th2，Th17/Treg subtype distribution.Results:The content of IL-1β and TNF-α in the peripheral blood of the model group significantly increased as compared with that in the blank group(P<0.01).The content of IL-1β and TNF-α in the peripheral blood of the Tuina group significantly decreased as compared with that in the model group(P<0.01).The PBMC apoptosis rate in the model group was significantly higher than that in the blank group(P<0.01)，while the PBMC apoptosis rate in the Tuina group was significantly lower than that in the model group(P<0.05).Compared with the blank group，the model group showed increased Th1 and Th17 cells in PBMCs，decreased Th2 and regulatory T(Treg) cells，and shifted Th1 and Th17 cells，resulting in Th1/Th2 and Th17/Treg cell ratio up-regulation(P<0.01).Compared with the model group，the Th1/Th2 and Th17/Treg cell ratios in PBMCs of the Tuina group were significantly down-regulated(P<0.01，P<0.05).Conclusion:Tuina can improve the immunoinflammatory status of peripheral blood in EF rats，showing the effects of anti-inflammatory，anti-apoptosis，and immune regulation. |
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