To investigate the intervention effect of Chaigui Decoction on blood pressure，levels of angiotensin-converting enzyme 2(ACE2) in plasma and the renal tissue，and intestinal flora in high-salt-induced hypertensive mice.Methods:In this study，a high-salt diet was used to induce a hypertension model in mice.Benazepril and Chaigui Decoction were administered in parallel for intervention，and the blood pressure，plasma ACE2 levels，renin and ACE2 levels in the renal tissue，and changes in intestinal flora abundance were measured.Results:1) Chaigui Decoction could continuously and stably reduce systolic and diastolic blood pressure levels in the high-salt-induced hypertensive mouse model(P<0.001)，with an antihypertensive effect comparable to that of benazepril(P>0.05). 2) Chaigui Decoction increased the decreased plasma ACE2 levels in hypertensive mice(P<0.001)，but the plasma ACE2 levels in the benazepril group were higher than those in the Chaigui Decoction group(P<0.001). 3) Chaigui Decoction reduced renal tissue renin levels and the renin/ACE2 ratio in hypertensive mice(P<0.05). 4) The abundance of Bacteroidia intestinal flora decreased，while Clostridia increased in hypertensive mice.Both benazepril and Chaigui Decoction improved the imbalance of intestinal flora(P<0.05).Conclusion:Chaigui Decoction has a good antihypertensive effect on high-salt-induced hypertensive mice，and its mechanism is related to the elevation of plasma ACE2 levels，reduction of renal tissue renin levels and renin/ACE2 ratio，and regulation of intestinal flora.