| 引用本文:徐英莉1，耿子涵1，于春江2，赵宏伟2，孙静1，包蕾1，赵荣华1，鲍岩岩1，曹姗1，庞博1，郭姗姗1，崔晓兰1.小儿热速清口服液防治柯萨奇A16型手足口病体内外作用研究[J].世界中医药,2023,(12):. |
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| 小儿热速清口服液防治柯萨奇A16型手足口病体内外作用研究 |
| Antiviral Effects of Xiao'er Resuqing Oral Liquid Against Coxsackievirus A16-induced HFMD in Vitro and in Vivo |
| 投稿时间：2022-04-04 |
| DOI：10.3969/j.issn.1673-7202.2023.12.007 |
| 中文关键词: 小儿热速清口服液 手足口病 柯萨奇病毒A16株 乳鼠模型 抗病毒 药效作用 预防性给药 治疗性给药 |
| English Keywords:Xiao'er Resuqing Oral Liquid Hand-foot-mouth disease Coxsackievirus A16 Sulking mouse model Antiviral Pharmacodynamic effect Prophylactic administration Therapeutic administration |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金资助项目（ZXKT21031）；中国中医科学院中医药防治流感技术体系项目（ZZ13-035-09） |
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| 中文摘要: |
| 目的：评价小儿热速清口服液对柯萨奇病毒A16株（Cox A16）体内外感染模型的抗病毒作用。方法：采用3日龄BALB/c乳鼠腹腔注射Cox A16，构建BALB/c乳鼠手足口病模型，设计了大、中、小3个剂量分别为62 mL/(kg·d)、31 mL/(kg·d)、15.5 mL/(kg·d)，进行预防性给药和治疗性给药评价。预防性给药实验中，观察记录乳鼠感染程度，存活天数，计算死亡率、生命延长率。治疗性给药实验中，采用实时聚合酶链反应（Real-time PCR）检测后肢肌肉病毒载量，苏木精-伊红染色（HE）观察后肢肌肉组织、脑组织病理病变情况。体外实验采用细胞病变效应法（CPE），其对Cox A16、Cox B4、Cox B5病毒的抑制作用。Real-time PCR法检测Cox A16体外病毒载量。结果：小儿热速清口服液高、中、小剂量组均可显著性延长存活天数（P<0.01或P<0.05），高、中剂量组可以显著降低死亡率和临床感染评分（P<0.01）。中、小剂量组均可以显著降低后肢肌肉病毒载量（P<0.01或P<0.05）。大、中显著改善后肢肌肉病理学改变及病理学评分（P<0.01或P<0.05），中剂量组显著改善脑组织病理学变化及病理学评分（P<0.05）。体外实验表明，小儿热速清口服液对肠道病毒Cox B5株、Cox A16株、Cox B4株具有明显的抑制作用。结论：小儿热速清口服液对肠道病毒COX A16感染BALB/c乳鼠模型具有良好的预防和治疗作用，可能通过抑制病毒复制发挥作用。 |
| English Summary: |
| To evaluate the antiviral effects of Xiao'er Resuqing Oral Liquid on coxsackievirus A16(Cox A16) infection models in vivo and in vitro.Methods:Three-day-old BALB/c mice were intraperitoneally injected with Cox A16 to establish a hand-foot-mouth disease(HFMD) model in BALB/c mice.Three different doses of Xiao'er Resuqing Oral Liquid ［62，31，and 15.5 mL/(kg·d)］ were designed for both prophylactic and therapeutic administration evaluations.In the prophylactic administration experiment，the infection level，survival days，mortality rate，and life extension rate of the mice were observed and recorded.In the therapeutic administration experiment，real-time polymerase chain reaction(PCR) was used to detect the viral load in the hind limb muscles，and hematoxylin-eosin(HE) staining was performed to observe the pathological changes in the hind limb muscle and brain tissues.In the in vitro experiment，the cytopathic effect(CPE) method was used to assess the inhibitory effects of Xiao'er Resuqing Oral Liquid on Cox A16，Cox B4，and Cox B5 viruses.Real-time PCR was used to detect the Cox A16 viral load in vitro.Results:Xiao'er Resuqing Oral Liquid at high，medium，and low doses significantly prolonged the survival days of the mice(P<0.01 or P<0.05).Xiao'er Resuqing Oral Liquid at high and medium doses significantly reduced the mortality rate and clinical infection score(P<0.01).Xiao'er Resuqing Oral Liquid at medium and low doses significantly reduced the viral load in the hind limb muscles(P<0.01 or P<0.05).Xiao'er Resuqing Oral Liquid at high and medium doses significantly improved the histopathological changes and pathological scores in the hind limb muscles(P<0.01 or P<0.05)，while that at medium dose significantly improved the histopathological changes and scores in the brain tissues(P<0.05).The in vitro experiment showed that Xiao'er Resuqing Oral Liquid had a significant inhibitory effect on Cox B5，Cox A16，and Cox B4 enteroviruses.Conclusion:Xiao'er Resuqing Oral Liquid has good prophylactic and therapeutic effects on the Cox A16 infection model in BALB/c mice and may exert its effects by inhibiting viral replication. |
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