To screen differential proteins in the trigeminal ganglia of migraine rats with phasic characteristics at different time points，analyze the related biological processes，and explore the changes in biological processes at different time points in the migraine with phasic characteristics rat model.Methods:The migraine with phasic characteristics model was established in rats by subcutaneous injection of dopamine and nitroglycerin.At different time points during the modeling process(11 min，22 min，and 33 min after dopamine injection; 1 h and 2 h after nitroglycerin injection)，the trigeminal ganglia were collected from the rats.Tandem mass tag(TMT) quantitative proteomics sequencing was performed to screen differential proteins at different time points，and the differentially expressed proteins were subjected to Gene Ontology(GO) functional annotation and enrichment analysis.Results:Compared with the blank control group，there were 1 323 differential proteins in the DA-11 group and 1 392 differential proteins in the DA-22 group，primarily related to biological processes such as oxidative stress response and metabolic processes.The DA-33 group had 961 differential proteins，primarily related to oxidative stress response and other biological processes.The GTN-1 group had 1 051 differential proteins，mainly associated with biological processes such as inflammation response and apoptosis.The GTN-2 group had 1 102 differential proteins，primarily related to metabolic processes.Key biological processes associated with contraction were mainly oxidative stress response and ATP metabolism，while key biological processes associated with relaxation were inflammation mediator regulation of TRP channels and cell adhesion.Conclusion:The molecular mechanism and biological processes in the rat model of migraine with phasic characteristics exhibit dynamic changes at different time points.The contraction phase is characterized by oxidative stress，while the relaxation phase is characterized by inflammation response and activation of TRP channels.These characteristics can provide a foundation for studying the dynamic pathological mechanisms of migraine and developing time-based precision treatments.