| 引用本文:张平1，李嘉琪1，刘雪峰1，潘广涛2，丁辛1，吕文亮1，刘菏婧1，许鹏1，王从安3.基于网络药理学及实验验证连朴饮治疗幽门螺杆菌阳性口周痤疮的作用机制[J].世界中医药,2023,(13):. |
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| 基于网络药理学及实验验证连朴饮治疗幽门螺杆菌阳性口周痤疮的作用机制 |
| Mechanism of Modified Lianpo Decoction in Treating Helicobacter pylori-positive Perioral Acne Based on Network Pharmacology and Experimental Verification |
| 投稿时间：2022-02-11 |
| DOI：10.3969/j.issn.1673-7202.2023.13.009 |
| 中文关键词: 连朴饮 网络药理学 口周痤疮 幽门螺杆菌 炎症 中西医结合 TOLL样受体4 核因子κB |
| English Keywords:Lianpu Decoction Network Pharmacology Perioral acne Helicobacter pylori Inflammation Integrated Chinese and Western medicine Toll-like receptor 4(TLR4) Nuclear factor-κB(NF-κB) |
| 基金项目:国家自然科学基金青年科学基金项目(82004495)；湖北中医药大学2022年青苗计划资助项目(2022ZXQ023) |
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| 中文摘要: |
| 目的：基于网络药理学和实验验证探讨连朴饮加减方治疗幽门螺杆菌（Hp）阳性口周痤疮的潜在靶点和作用机制。方法：利用网络药理学工具，获得止嗽散治疗慢性支气管炎的有效成分、核心靶点和信号通路。采用Hp菌液灌服法联合皮内注射痤疮丙酸杆菌构建Hp阳性口周痤疮小鼠模型，采用苏木精-伊红(HE)染色小鼠口周皮肤组织，采用酶联免疫吸附试验(ELISA)测定各组小鼠血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平；采用蛋白质印迹法检测小鼠口周皮肤组织中NOD1、Toll样受体(TLR4)、核因子κB蛋白表达。结果:筛选得到连朴饮关键靶点42个，主要涉及信号转导及转录激活因子（STAT3)、促分裂原活化的蛋白激酶（MAPK1）等；基因本体（GO）功能富集分析结果共477个生物过程；京都基因和基因组百科全书（KEGG）通路富集分析结果共有102条通路。动物实验表明，与空白对照组比较，模型对照组小鼠血清TNF-α、IL-6水平显著升高(均P<0.01)，口周皮肤组织中NOD1、TLR4、核因子κB蛋白表达显著升高；与模型对照组比较，连朴饮加减方组及中西医结合组小鼠血清TNF-α、IL-6水平显著降低(均P<0.01)，口周皮肤组织中NOD1、TLR4、核因子κB蛋白表达显著下降(均P<0.01)。结论：连朴饮治疗Hp阳性痤疮具有多成分、多靶点、多通路协同作用，其作用机制可能与调控TLR4/核因子κB信号通路有关。 |
| English Summary: |
| To explore the potential targets and mechanism of modified Lianpu Decoction in treating Helicobacter pylori(Hp)-positive perioral acne based on network pharmacology and experimental verification.Methods:The effective components，core targets，and signaling pathways of modified Lianpu Decoction in treating Hp-positive perioral acne were obtained by network pharmacology.The mouse model of Hp-positive perioral acne was established by gavage with Hp suspension combined with intracutaneous injection of Propionibacterium acnes.The perioral skin tissue samples of the mice were stained with hematoxylin-eosin(HE)，and serum levels of tumor necrosis factor(TNF-α) and interleukin(IL-6) were determined by enzyme-linked immunosorbent assay(ELISA).The protein levels of nucleotide-binding oligomerization domain 1(NOD1)，Toll-like receptor(TLR4)，and nuclear factor κB(NF-κB) in the perioral skin were determined by Western blotting.Results:A total of 42 key targets of Lianpu Decoction were selected，mainly involving signal transducer and activator of transcription 3(STAT3) and mitogen-activated protein kinase 1(MAPK1).The gene ontology(GO) annotation predicted 477 biological processes.The Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis showed that the key targets were involved in 102 signaling pathways.The animal experiments showed that compared with the blank control group，the modeling elevated the serum levels of TNF-α and IL-6(P<0.01) and up-regulated the expression levels of NOD1，TLR4，and NF-κB in the perioral skin.Compared with the model group，modified Lianpu Decoction and integrated Chinese and Western medicine lowered the serum levels of TNF-α and IL-6(P<0.01) and down-regulated the expression levels of NOD1，TLR4，and NF-κB in the perioral skin(P<0.01).Conclusion:Lianpu Decoction can treat Hp-positive acne in a multi-component，multi-target，and multi-pathway manner by regulating the TLR4/NF-κB signaling pathway. |
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