世界中医药

目的：观察补肾生血药对顺铂损伤的骨髓间充质干细胞（BMSC）的影响，探讨补肾生血改善化疗后骨髓抑制的机制。方法：将50只SD雄性大鼠随机分为空白对照组、补肾生血药组，每组25只，分别制备补肾生血血清和空白血清，原代培养BALB/c小鼠BMSC，随机分组，CCK-8法确定顺铂和补肾生血血清干预浓度。干预48 h后，采用流式细胞仪检测细胞凋亡率和细胞周期，酶联免疫吸附试验法检测培养液中造血调控因子含量。结果：干预48 h后，骨髓细胞凋亡率明显增高（P<0.01），补肾生血血清组G1期细胞比例显著降低（P<0.05），S期细胞比例显著增加（P<0.05），增殖期细胞比例明显增加（P<0.05）。补肾生血血清组骨髓细胞凋亡率明显降低（P<0.001），G1期细胞比例显著降低（P<0.05），增殖期细胞比例显著增加（P<0.05），IL-3含量明显升高（P<0.01），TNF-α、TGF-β、IFN-γ含量显著降低（均P<0.05）。结论：补肾生血药能减轻顺铂造成的BMSC凋亡，促进细胞进入增殖期，促进造血生长因子分泌，减少造血抑制因子分泌，提示该药对化疗后骨髓抑制具有一定的改善作用。

English Summary:

To observe the effect of Bushen Shengxue Formula on cisplatin-induced damage to bone marrow mesenchymal stem cells(BMSCs) and explore the mechanism of Bushen Shengxue Formula in improving bone marrow suppression after chemotherapy.Methods:Fifty male SD rats were randomly divided into a blank control group and a Bushen Shengxue Formula group，with 25 rats in each group.Bushen Shengxue Formula-medicated serum and blank serum were prepared，and primary cultures of BALB/c mouse BMSCs were established.BMSCs were randomly divided into groups，and the intervention concentrations of cisplatin and Bushen Shengxue Formula-medicated serum were determined using the CCK-8 method.After 48 hours of intervention，flow cytometry was used to detect cell apoptosis rate and cell cycle，and the enzyme-linked immunosorbent assay was used to measure the levels of hematopoietic regulatory factors in the culture medium.Results:After 48 hours of intervention，the apoptosis rate of bone marrow cells increased significantly(P<0.01).In the Bushen Shengxue Formula-medicated serum group，the proportion of cells in the G1 phase decreased significantly(P<0.05)，the proportion of cells in the S phase increased significantly(P<0.05)，and the proportion of proliferating cells increased significantly(P<0.05).The Bushen Shengxue Formula-medicated serum group showed a significant decrease in the apoptosis rate of bone marrow cells(P<0.001)，a significant decrease in the proportion of cells in the G1 phase(P<0.05)，a significant increase in the proportion of proliferating cells(P<0.05)，and a significant increase in IL-3 level(P<0.01)，and decreased levels of TNF-α，TGF-β，and IFN-γ(all P<0.05).Conclusion:Bushen Shengxue Formula can alleviate cisplatin-induced apoptosis of BMSCs，promote cell proliferation，stimulate the secretion of hematopoietic growth factors，and reduce the secretion of hematopoietic inhibitory factors.These results suggest that this formula has a certain improving effect on bone marrow suppression after chemotherapy.

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