| 引用本文:阿丽亚·依拉木，白姣姣，阿布都艾则孜·艾尔肯，艾尔菲丁·艾尼瓦尔，木巴拉克·伊明江.没食子体外抗结肠癌活性提取物的筛选研究[J].世界中医药,2023,(13):. |
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| 没食子体外抗结肠癌活性提取物的筛选研究 |
| In Vitro Screening of Anti-colorectal Cancer Extracts from Quercus infectoria Galls |
| 投稿时间：2021-10-24 |
| DOI：10.3969/j.issn.1673-7202.2023.13.012 |
| 中文关键词: 没食子 没食子酸 5-氟尿嘧啶 不同极性 溶剂 提取物 结肠癌 抑制 增殖 |
| English Keywords:Quercus.infectoria galls Gallic acid 5-fluorouracil Different polarities Solvent Extract Colorectal cancer Inhibit Proliferation |
| 基金项目:新疆维吾尔自治区自然科学基金面上项目（2021D01C259） |
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| 中文摘要: |
| 目的：采用不同极性溶剂提取回流得到4种没食子（QIG）提取物，筛选出最佳抑制结肠癌细胞活性的没食子提取物。方法：8倍溶剂提取回流3次，每次0.5 h，浓缩蒸干制备没食子丙酮、乙醇、甲醇、水提部位浸膏；以化疗药物5-氟尿嘧啶为阳性对照药，CCK-8法测定有效提取物和没食子酸在不同浓度以及不同作用时间孵育之后对结肠癌HCT-116、Caco-2细胞的体外抑制作用。结果：没食子4种提取物和没食子酸对2种结肠癌细胞均有不同程度的抑制作用。没食子4种提取物在12、24、48、72 h对HCT-116细胞的半数抑制浓度（IC50值）范围分别为：0.313~0.988 mg/mL、0.131~0.53 mg/mL、0.107~0.379 mg/mL、0.1~0.262 mg/mL；对Caco-2细胞分别为1.093~1.382 mg/mL、0.404~0.53 mg/mL、0.246~0.341 mg/mL、0.134~0.312 mg/mL；综合比较可知其中乙醇提取物对2种细胞活性抑制效果最好，其在作用72 h时HCT-116和Caco-2细胞对应的最大抑制率和IC50值分别为76.55%和0.1 mg/mL、78.8%和0.134 mg/mL。没食子酸在作用72 h时HCT-116和Caco-2细胞对应的最大抑制率和IC50值分别为86.54%和0.07 mg/mL、80.3%和0.083 mg/mL。结论：没食子不同极性溶剂提取物对结肠癌HCT-116、Caco-2细胞具有不同程度的抑制作用，提取物当中没食子乙醇提取物相对其他提取物对2种结肠癌细胞抑制效果最佳。 |
| English Summary: |
| To extract four types of Quercus infectoria galls(QIG) extracts using solvents with different polarities and screen for the optimal QIG extract with the highest inhibitory activity against colon cancer cells.Methods:QIG extracts were obtained by reflux extraction for three times using eight-fold solvents，with each extraction performed for 0.5 hours.The resulting extracts were concentrated and dried to prepare QIG extracts in acetone，ethanol，methanol，and water.The chemotherapy drug 5-fluorouracil was used as a positive control.The CCK-8 assay was used to evaluate the inhibitory effects of the active extracts and gallic acid at different concentrations and incubation periods on HCT-116 and Caco-2 colon cancer cells in vitro.Results:The four QIG extracts and gallic acid exhibited varying degrees of inhibitory effects on the two colon cancer cell lines.The IC50 values of the four extracts against HCT-116 cells at 12，24，48，and 72 hours were 0.313～0.988 mg/mL，0.131~0.53 mg/mL，0.107~0.379 mg/mL，and 0.1~0.262 mg/mL，respectively.For Caco-2 cells，the IC50 values were 1.093~1.382 mg/mL，0.404~0.53 mg/mL，0.246～0.341 mg/mL，and 0.134~0.312 mg/mL，respectively.Among them，the ethanol extract showed the best inhibitory effect on both cell lines.At 72 hours，the maximum inhibition rates and IC50 values of the ethanol extract on HCT-116 and Caco-2 cells were 76.55% and 0.1 mg/mL，and 78.8% and 0.134 mg/mL，respectively.Gallic acid showed maximum inhibition rates and IC50 values of 86.54% and 0.07 mg/mL，and 80.3% and 0.083 mg/mL，respectively，at 72 hours.Conclusion:QIG extracts obtained using solvents with different polarities exhibited varying degrees of inhibitory effects on HCT-116 and Caco-2 colon cancer cells.Among the extracts，QIG ethanol extract showed the most promising inhibitory effects on both cell lines. |
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