| 引用本文:魏路路1，郭书文1，2，力一凡1，冯鹏飞1.基于参七汤的人参-三七药对治疗心肌梗死疗效的荟萃分析及网络药理学研究[J].世界中医药,2023,(14):. |
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| 基于参七汤的人参-三七药对治疗心肌梗死疗效的荟萃分析及网络药理学研究 |
| Meta-analysis and Network Pharmacology on the Efficacy of Ginseng Radix et Rhizoma-notoginseng Radix et Rhizoma Herbal Pair in Shenqi Decoction in the Treatment of Myocardial Infarction |
| 投稿时间：2021-10-14 |
| DOI：10.3969/j.issn.1673-7202.2023.14.015 |
| 中文关键词: 心肌梗死 临床疗效 活性成分 靶点 通路 荟萃分析 网络药理学 |
| English Keywords:Myocardial infarction Clinical effectiveness Active ingredient Target Pathway Meta-analysis Network pharmacology |
| 基金项目:国家自然科学基金项目（81774031）；北京市自然科学基金项目（7202119） |
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| 中文摘要: |
| 目的：系统评价参七汤治疗心肌梗死（MI）的临床疗效，通过网络药理学阐述人参-三七药对对心肌梗死的作用机制。方法：检索国家知识基础设施数据库（CNKI）、中国生物医学文献数据库（CBM）、中国学术期刊数据库（CSPD）及中文科技期刊数据库（CCD）、PubMed、CochraneLibrary、Embase数据库自建库至2022年12月发表的相关文献，经文献筛选、质量评估、数据提取进行Meta分析，查询中药系统药理学数据库与分析平台（TCMSP）获取并筛选人参、三七的活性成分及其靶点，从GeneCards、OMIM、DRUGBANK、TTD数据库获取心肌梗死的相关靶点，将获得的数据导入String数据库构建蛋白质-蛋白质相互作用（PPI）网络，使用Cytoscape 3.7.1构建成分-靶点网络和成分-靶点-通路网络，通过Metascape数据库进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）通路富集分析，最后利用AutoDock 4.2进行分子对接验证。结果：共纳入9项研究，650例患者.与西医常规观察组比较，参七汤联合常规观察组可减少左室舒张末期内径、低密度脂蛋白，提高射血分数及临床总有效率。网络药理学提示人参-三七作用于MI的活性成分有26种，包含高丽槐素、原阿片碱、槲皮素、豆甾醇、β-谷甾醇等，人参-三七作用于MI的潜在靶点有102个，包含XDH、VCAM1、TNF、TGFB1等，人参-三七调节MI的生物学通路为AGE-RAGE信号通路、PI3K-AKT信号通路、核因子κB信号通路、HIF-1信号通路、cGMP-PKG信号通路,分子对接结果显示人参-三七的主要活性成分槲皮素、山柰酚、β-谷甾醇与靶点ADRB2结合较好。结论：参七汤联合西医常规治疗可提高心肌梗死后心室重构的临床疗效，推测人参-三七主要通过调节线粒体功能、氧化应激、钙稳态、炎症反应以及抑制细胞凋亡等治疗MI，为进一步的实验研究提供一定的理论支持。 |
| English Summary: |
| To systematically evaluate the clinical efficacy of Shenqi Decoction in treating myocardial infarction(MI) and elucidate the pharmacological mechanism of Ginseng Radix et Rhizoma-Notoginseng Radix et Rhizoma Herbal Pair on MI using network pharmacology.Methods:Relevant research articles were retrieved from databases including the National Knowledge Infrastructure(CNKI)，China Biology Medicine(CBM)，China Science Periodical Database(CSPD)，Chinese Citation Database(CCD)，PubMed，Cochrane Library，and Embase from database inception to December 2022.After literature screening，quality assessment，and data extraction，Meta-analysis was conducted.Active ingredients and targets of Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).MI-related targets were obtained from GeneCards，OMIM，DRUGBANK，and TTD databases.The data were imported into the String database to construct the protein-protein interaction(PPI) network.Cytoscape 3.7.1 was used to construct ingredient-target and ingredient-target-pathway networks.Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analyses were performed using the Metascape database，followed by molecular docking validation using AutoDock 4.2.Results:Nine studies involving 650 patients were included.Compared with the conventional western medicine group，Shenqi Decoction combined with conventional treatment reduced left ventricular end-diastolic diameter and low-density lipoprotein and increased ejection fraction and overall clinical effective rate.Network pharmacology indicated that Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma had 26 active ingredients，including maackiain，fumarine，quercetin，stigmasterol，and β-sitosterol.Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma targeted 102 potential targets related to MI，including XDH，VCAM1，TNF，and TGFB1.The biological pathways regulated by Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma in MI treatment were found to be the AGE-RAGE signaling pathway，PI3K-AKT signaling pathway，nuclear factor kappa B(NF-κB) signaling pathway，HIF-1 signaling pathway，and cGMP-PKG signaling pathway.The molecular docking results showed that the main active ingredients quercetin，kaempferol，and β-sitosterol had good binding affinity with the target ADRB2.Conclusion:Shenqi Decoction combined with conventional western medicine treatment can improve clinical efficacy in ventricular remodeling after MI.It is inferred that Ginseng Radix et Rhizoma and Notoginseng Radix et Rhizoma mainly treat MI by regulating mitochondrial function，oxidative stress，calcium homeostasis，inflammatory responses，and inhibiting cell apoptosis，providing theoretical support for further experimental research. |
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