To establish the method for the quality control of the standard decoctions prepared from the powder of Asari Radix et Rhizoma.Methods:According to the preparation method of standard decoctions of Chinese medicinal material powder，the decoctions were prepared from the powder of Asari Radix et Rhizoma samples produced in different areas.High performance liquid chromatography(HPLC) was employed to determine the content，yield，and transfer rate of asarinin in the decoctions，and the HPLC fingerprint was established.Furthermore，the main components in the decoctions were identified by ultra-performance liquid chromatography-quadrupole time of flight-mass spectrometry(UPLC-QTOF-MS) for the main peaks.Results:Asarinin showed a good linear relationship within the tested concentration range.The methodological investigation showed that the relative standard deviations of the stability，precision，and repeatability were all less than 3%.The transfer rate ranged from 9.25% to 11.0%，and the yield from 25.76 mg/mL to 28.25 mg/mL.The fingerprint similarity of the decoctions prepared from the powder of Asari Radix et Rhizoma was greater than 90.0%.A total of 16 compounds were identified，including aristolochic acid I，aristolochic acid D，and aristolactam I.Conclusion:The quality evaluation method for the standard decoctions prepared from the powder of Asari Radix et Rhizoma was established.