The action mechanism of Xiang Sha Zhi Zhu Wan(XSZZW) in the treatment of functional dyspepsia(FD) was explored by network pharmacology and verified by animal experiments.Methods:The effective components and targets of XSZZW were screened through traditional Chinese medicines systems pharmacology platform(TCMSP) and a bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine(BATMAN-TCM).The targets of components were standardized by the Perl language and the Uniprot database.The target of FD was screened by the Genecards，OMIM，Drugbank，as well as TTD databases.By using Cytoscape，the network of Chinese medicine-effective components-corresponding targets was constructed.The protein-protein interaction(PPI) networks of corresponding targets of effective components of XSZZW and FD-related targets were constructed by STRING database，respectively.Then，the PPI networks were imported to Cytoscape.Based on the values of degree，closeness centrality，and betweenness centrality，key targets were collected and mapped to the DAVID database.Gene ontology(GO) enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis were conducted in the DAVID database.Finally，the results were validated by molecular docking and animal experiments.Results:A total of 75 effective components of XSZZW，292 corresponding targets，666 FD-related targets，and 90 key targets were obtained finally.GO enrichment analysis mainly included identical protein binding，enzyme binding，protein binding，and other biological regulation.103 pathways were enriched by KEGG analysis，mainly related to cancer，apoptosis，inflammation pathways，and other pathways.Conclusion:XSZZW can prevent and treat FD by regulating PI3K/AKT signaling pathways，FoxO signaling pathways，NF-κB signaling pathways，and neurotrophic factor signaling pathways.