The study aims to analyze the action mechanism of Cangerzi Powder in the treatment of seasonal allergic rhinitis based on network pharmacology and provide references for the clinical expansion of the classic prescriptions and the evaluation of the efficacy.Methods:The main chemical components and targets of Fructus Xanthii，Flos Magnoliae，Radix Angelicae Dahuricae，and Rhizoma Ligustici Chuanxiong were obtained from the traditional Chinese medicines systems pharmacology platform(TCMSP)，and the active components of traditional Chinese medicine were screened according to absorption，distribution，metabolism，and excretion(ADME).The network imaging software Cytoscape 3.7.2 was used to analyze the active components and target networks of Cangerzi Powder.Corresponding targets of seasonal allergic rhinitis were obtained through the Gencards database.Cytoscape 3.7.2 was utilized to construct a protein-protein interaction(PPI) network，and the key targets extracted from the network were submitted to the STRING11.0 database to analyze the biological processes involved.After taking the intersection genes of drug targets and disease targets，the database for annotation，visualization，and integrated discovery(DAVID database) was used to perform gene ontology(GO) enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis，and the “component-target-pathway” network was built.The screened targets were verified by enzyme-linked immunosorbent assay(ELISA) by constructing an ovalbumin(VOA) rat model of allergic rhinitis.Results:The core active components of Cangerzi Powder in the treatment of seasonal allergic rhinitis were naringin，stigmasterol，luteolin，etc.The core targets were PTGS2，PIK3CG，PTGS1，AKT1，TNF，ADRB2，EGFR，IL-1β，etc.The biological pathway of Cangerzi Powder in the treatment of seasonal allergic rhinitis mainly acted on the calcium signaling pathway，HIF-1 signaling pathway，Toll-like receptor signaling pathway，TNF signaling pathway，etc.Its function was mainly to regulate the expression of inflammation and immune-related cytokines.The results of animal experiments demonstrated that compared with the normal control group，the serum levels of IgE，IL-4，PTGS2，PTGS1，and AKT1 mRNA expressions were significantly increased(P<0.01) in the model control group，while the mRNA expression of interferon-γ(IFN-γ) was significantly decreased(P<0.01).Furthermore，compared with the model control group，the mRNA expressions of IgE，IL-4，PTGS2，PTGS1，and AKT1 were significantly decreased(P<0.01)，and the mRNA expression of IFN-γ was significantly increased(P<0.05).Conclusion:This study utilizes network pharmacology to investigate the efficacy of Cangerzi Powder in treating seasonal allergic rhinitis.The findings suggest that Cangerzi Powder acts through multiple targets and pathways.The rat model of allergic rhinitis is used to confirm that the mRNA expression of PTGS2，PTGS1，and AKT1 significantly decreased after treatment with Cangerzi Powder，indicating that these may be key targets of Cangerzi Powder in the treatment of seasonal allergic rhinitis.