| 引用本文:韩亚光1，宋思钰2，孙小惠2，韩延华2，朱小琳3.针刺疗法对子宫内膜异位症大鼠细胞凋亡的影响[J].世界中医药,2023,(16):. |
|
| 针刺疗法对子宫内膜异位症大鼠细胞凋亡的影响 |
| Effect of Acupuncture on Apoptosis in Rats with Endometriosis |
| 投稿时间：2022-09-26 |
| DOI：10.3969/j.issn.1673-7202.2023.16.006 |
| 中文关键词: 子宫内膜异位症 针刺疗法 细胞凋亡 PI3K/AKT/mTOR信号通路 异位增生 胱天蛋白酶-3 Bcl-2相关X蛋白 雌激素 |
| English Keywords:Endometriosis Acupuncture Apoptosis PI3K/AKT/mTOR signaling pathway Ectopic hyperplasia Cysteine aspartate protease-3 Bcl-2-associated X protein Estrogen |
| 基金项目:国家自然科学基金项目（81574015）；2020博士后科研启动金资助项目（LBH-Q20057）；2021博士后科研启动金资助项目（LBH-Q21187） |
|
| 摘要点击次数: 591 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：基于磷脂酰肌醇3激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白（PI3K/AKT/mTOR）信号通路，探究针刺疗法对子宫内膜异位症（EMs）大鼠异位内膜组织细胞凋亡的影响及相关可能机制。方法：采用自体移植法构建EMs动物模型，按照随机数字表法分为模型组、针刺组、对照组，每组12只。针刺组给予针刺治疗，1次/d，连续治疗28 d，对照组和模型组不给予任何治疗。采用苏木精-伊红染色法（HE染色）观察大鼠内膜组织病理形态；原位末端凋亡法（TUNEL）观察在位及异位内膜组织细胞凋亡变化；采用荧光定量PCR（RT-PCR）和蛋白质印迹法检测在位及异位内膜组织雌激素受体α（ER-α）、雌激素受体β（ER-β)、PI3K、AKT、mTOR、胱天蛋白酶-3(Caspase-3）、Bcl-2相关X蛋白（Bax）和B细胞淋巴瘤-2（Bcl-2)的基因表达和蛋白含量变化。结果：与对照组比较，模型组异位组织有明显异位增生和炎症介质浸润，PI3K、AKT、mTOR基因表达和蛋白含量显著升高(P<0.05)；治疗干预后，与模型组比较，针刺组异位组织中，细胞增殖和炎症介质浸润明显缓解，PI3K、AKT、mTOR、Bcl-2基因表达和蛋白含量均降低，而Caspase-3、Bax基因表达和蛋白含量明显升高(P<0.05)。结论：针刺治疗可加速异位病灶组织中的细胞凋亡，这可能与调控PI3K/AKT/mTOR信号通路促进相关凋亡因子密切相关。 |
| English Summary: |
| Based on the phosphatidylinositol3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR) signaling pathway，the effect of acupuncture on apoptosis of ectopic endometrial histiocytes in rats with endometriosis(EMs) and related mechanisms were explored.Methods:The animal model of EMs was constructed by autologous transplantation，which was divided into the model group，control group，and acupuncture group according to the random number table method，with 12 animals in each grou.The acupuncture group was given acupuncture once a day for 28 days，and no treatment regimen was given to the control group and the model group.The histopathological morphology of the intima of rats was observed by hematoxylin-eosin(HE) staining.TdT-mediated dUTP nick-end labeling(TUNEL) was used to observe apoptosis changes in in-situ and ectopic endometrial histiocytes.Real-time polymerase chain reaction(RT-PCR) and western blot were used to detect gene expression and protein content changes in estrogen receptor α(ER-α)，estrogen receptor β(ER-β)，PI3K，AKT，mTOR，cysteine aspartate protease-3(Caspase-3)，Bcl-2-associated X protein(Bax)，and B lymphocyte tumor 2(Bcl-2) in in-situ and ectopic endometrial tissue.Results:Compared with the control group，the model group had significant ectopic hyperplasia and inflammatory factor infiltration，and the expression and protein content of PI3K，AKT，and mTOR genes were significantly increased(P<0.05).After the treatment intervention，compared with the model group，cell proliferation and inflammatory factor infiltration were significantly alleviated，while the gene expression and protein content of PI3K，AKT，mTOR，and Bcl-2 were significantly reduced，and those of Caspase-3 and Bax were significantly increased(P<0.05).Conclusion:Acupuncture can accelerate apoptosis in ectopic lesion tissue，which may be closely related to the regulation of the PI3K/AKT/mTOR signaling pathway to promote related apoptotic factors. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
