| 引用本文:黎发根1，王亮1，李绍旦1，杨明会1，周建平2，刘毅1.和胃安神方对失眠模型大鼠5-羟色胺、多巴胺及去甲肾上腺素水平的影响[J].世界中医药,2023,(16):. |
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| 和胃安神方对失眠模型大鼠5-羟色胺、多巴胺及去甲肾上腺素水平的影响 |
| Effect of Hewei Anshen Formula on Levels of 5-HT，DA，and NE in Insomnia Model Rats |
| 投稿时间：2021-06-08 |
| DOI：10.3969/j.issn.1673-7202.2023.16.007 |
| 中文关键词: 和胃安神方 中医药疗法 失眠 动物模型 5-羟色胺 多巴胺 去甲肾上腺素 |
| English Keywords:Hewei Anshen Formula Chinese Medical therapy Insomnia Animal model 5-HT DA NE |
| 基金项目:国家自然科学基金项目（82174283） |
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| 中文摘要: |
| 目的：探讨和胃安神方对失眠模型大鼠下丘脑5-羟色胺（5-HT）、多巴胺（DA）和去甲肾上腺素（NE）水平的影响。方法：将60只雄性Wistar大鼠随机分为6组，即正常对照组、失眠模型组、地西泮组、和胃安神方低剂量组、和胃安神方中剂量组、和胃安神方高剂量组。采用酶联免疫吸附试验（ELISA）检测各组大鼠下丘脑组织5-HT、DA、NE的水平。结果：与正常对照组比较，失眠模型组大鼠5-HT水平显著下降，差异有统计学意义（P<0.05)，DA、NE水平较正常对照组显著升高，差异有统计学意义（P<0.05)；与失眠模型组比较，地西泮组、和胃安神方低剂量组5-HT、DA、NE水平差异均无统计学意义(均P>0.05)；和胃安神方中剂量组、和胃安神方高剂量组5-HT水平均较失眠模型组显著升高，差异有统计学意义(P<0.05)，DA、NE水平较失眠模型组显著下降，差异有统计学意义（P<0.05)。结论：和胃安神方治疗失眠症的疗效机制可能与调节脑内5-HT、DA、NE水平有关。 |
| English Summary: |
| To investigate the effects of Hewei Anshen Formula(HWAS) on the levels of 5-hydroxytryptamine(5-HT)，dopamine(DA)，and norepinephrine(NE) in the hypothalamus of insomnia model rats.Methods:Sixty male Wistar rats were randomly divided into six groups：a normal control group，an insomnia model group，a diazepam group，and low-，medium-，and high-dose HWAS groups.Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of 5-HT，DA，and NE in the hypothalamic tissues of the rats in each group.Results:The 5-HT level in the hypothalamus of rats in the insomnia model group was significantly decreased(P<0.05)，while the levels of DA and NE were significantly increased(P<0.05) as compared with those in the normal control group.There were no significant differences in 5-HT，DA，and NE levels in the diazepam group and the low-dose HWAS group when compared with the insomnia model group(P>0.05).The medium-dose and high-dose HWAS groups showed significantly higher 5-HT levels than the insomnia model group(P<0.05)，while the levels of DA and NE were significantly decreased(P<0.05).Conclusion:The therapeutic mechanism of the HWAS for treating insomnia may be related to the regulation of 5-HT，DA，and NE levels in the brain. |
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