| 引用本文:李文昊1，任鹏鹏2，韩洁茹1，常佳怡1，解颖1，陈飞1，李富震1，姜德友1.基于嘌呤配体P2X门控离子通道型受体7的资生肾气丸镇痛机制研究[J].世界中医药,2023,(17):. |
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| 基于嘌呤配体P2X门控离子通道型受体7的资生肾气丸镇痛机制研究 |
| Zisheng Shenqi Pills Relieve Pain via Purinergic Receptor P2X，Ligand-gated Ion Channel 7 |
| 投稿时间：2021-12-14 |
| DOI：10.3969/j.issn.1673-7202.2023.17.008 |
| 中文关键词: 资生肾气丸 镇痛机制 嘌呤配体P2X门控离子通道型受体7 Nod样受体蛋白3 NIMA相关激酶7 前列腺素E2 白细胞介素-1β 痛风性关节炎 |
| English Keywords:Zisheng Shenqi Pills Pain-relieving mechanism purinergic receptor P2X,ligand-gated ion channel 7 Nod-like receptor protein 3 NIMA-related kinase 7 Prostaglandin E2 Interleukin-1β Gouty arthritis |
| 基金项目:黑龙江省中医药科研项目（ZYW2022-047）；黑龙江中医药大学校基金项目（201733）；黑龙江中医药大学基础医学院青年教师科研资助项目（JCQNZR-Ⅱ-05） |
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| 中文摘要: |
| 目的：探索资生肾气丸对醋酸致小鼠扭体模型的镇痛作用。方法：将42只小鼠随机分为7组，分别给予相应药液灌胃，末次灌胃1 h后造模，建立醋酸致小鼠扭体模型。观察小鼠扭体次数，计算其扭体抑制率，应用酶联免疫吸附试验(ELISA)检测各组小鼠血清白细胞介素-1β(IL-1β）、前列腺素E2（PGE2）、神经生长因子（NGF）含量，采用实时PCR(RT-PCR)检测嘌呤配体P2X门控离子通道型受体7（P2X7R）mRNA、Nod样受体蛋白3（NLRP3）mRNA、NIMA相关激酶7（NEK7）mRNA的表达情况。结果：与模型组比较，随着时间的增加，资生肾气丸低剂量、中剂量、高剂量均可抑制小鼠扭体反应，降低扭体次数，增加小鼠扭体抑制率，降低小鼠血清中IL-1β、PGE2、NGF的含量，降低P2X7RmRNA、NLRP3mRNA、NEK7mRNA的表达以抑制疼痛反应，以高剂量效果最优（P<0.05），与吲哚美辛、痛风舒片疗效相近。结论：资生肾气丸高剂量对小鼠血清疼痛因子的降低效果显著，其机制可能与嘌呤配体P2X门控离子通道型受体7信号通路有关。 |
| English Summary: |
| To explore the pain-relieving effect of Zisheng Shenqi Pills on the mouse model of writhing induced by acetic acid.Methods:Forty-two mice were randomized into 7 groups and administrated with corresponding drug solutions by gavage.The model was established 1 h after the last administration.The writhing times of mice were observed，and the writhing inhibition rate was calculated.The enzyme-linked immunosorbent assay(ELISA) was employed to measure the levels of interleukin-1β(IL-1β)，prostaglandin E2(PGE2)，and nerve growth factor(NGF) in the serum.The mRNA levels of purinergic receptor P2X，ligand-gated ion channel 7(P2X7R)，Nod-like receptor protein 3(NLRP3)，and NIMA-related kinase 7(NEK7) were determined by RT-PCR.Results:Compared with the model group，Zisheng Shenqi Pills at low，medium，and high doses inhibited the writhing response，reduced the writhing times，increased the inhibition rate of writhing，and lowered the levels of IL-1β，PGE2，and NGF in the serum of mice.Moreover，they down-regulated the mRNA levels of P2X7R，NLRP3，and NEK7 to inhibit the pain response.The high dose group demonstrated the best effect(P<0.05)，which was similar to that of indometacin and Tongfengshu Tablets.Conclusion:Zisheng Shenqi Pills at high doses can significantly lower the levels of pain factors in the serum of mice by regulating the P2X7R signaling pathway. |
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