| 引用本文:林淑芳1,2，王嘉驹3，陈白1,2，曹丽萍1,2，姚娟娟1,2，江一静1,2.针刺太冲穴对肝阳上亢型偏头痛大鼠血清血管活性物质含量的影响[J].世界中医药,2023,(18):. |
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| 针刺太冲穴对肝阳上亢型偏头痛大鼠血清血管活性物质含量的影响 |
| Effects of Acupuncture at Taichong on Serum Vasoactive Substances in Migraine Rats with Ascendant Hyperactivity of Liver Yang |
| 投稿时间：2022-10-28 |
| DOI：10.3969/j.issn.1673-7202.2023.18.004 |
| 中文关键词: 偏头痛 肝阳上亢 针刺 太冲穴 血管活性物质 降钙素基因相关肽 5-羟色胺 肿瘤坏死因子-α |
| English Keywords:Migraine Ascendant hyperactivity of liver yang Acupuncture Taichong(LR 3) Vasoactive substances Calcitonin gene-related peptide(CGRP) 5-Hydroxy tryptamine(5-HT) Tumor necrosis factor-α(TNF-α) |
| 基金项目:国家自然科学基金青年科学基金项目（81904268）；福建省卫健委科技计划项目（2020QNA068） |
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| 中文摘要: |
| 目的：探讨针刺太冲穴对肝阳上亢型偏头痛模型鼠的作用机制。方法：将50只SD大鼠随机分为空白对照组（n=10）、模型组（n=40），模型组大鼠以附子汤灌胃结合颈肩部皮下注射硝酸甘油建立肝阳上亢偏头痛模型，其中30只大鼠成模，将成模的大鼠随机分为模型组、太冲组、非穴组，每组10只。太冲组针刺双侧太冲穴，非穴组针刺双侧肋下非穴，留针30 min。观察大鼠行为学（耳红持续时间、挠头及爬笼次数）、酶联免疫吸附试验法检测大鼠血清降钙素基因相关肽（CGRP）、5-羟色胺（5-HT）、肿瘤坏死因子-α（TNF-α）含量。结果：模型组、太冲组及非穴组均在注射硝酸甘油后4 min左右耳红，持续3 h左右消失；与模型组、非穴组比较，太冲组大鼠耳红持续时间较短（均P<0.05）。针刺前，除空白对照组外，大鼠的挠头、爬笼次数增加且组间差异无统计学意义（P>0.05）；针刺后1 h、2 h，与模型组和非穴组比较，太冲组大鼠挠头、爬笼次数均减少（均P<0.05）。与空白对照组比较，模型组大鼠血清中CGRP、TNF-α含量增高(P<0.05)，5-HT含量降低(P<0.05)；与模型组和非穴组比较，太冲组大鼠血清中CGRP、TNF-α含量减少(均P<0.05)，5-HT含量增多(均P<0.05)。结论：针刺双侧太冲穴可缓解偏头痛，其作用机制可能与降低血清中CGRP、TNF-α含量，提高5-HT含量有关。 |
| English Summary: |
| To explore the mechanism of acupuncture at Taichong(LR 3) on migraine rats with ascendant hyperactivity of liver yang.Methods:Fifty rats were randomly divided into blank control group(n=10) and model group(n=40).Rats in model group received modeling of migraine with ascendant hyperactivity of liver yang by gavage with Fuzi Decoction and subcutaneous injection of nitroglycerin.Thirty rats were successfully modeled and randomly divided into modeled group，LR 3 group and non-acupoint group，10 in each group.LR 3 group was treated with acunpuncture at bilateral LR 3 while non-acupoint group received acupuncture at bilateral subcostal non-acupoint both for 30 min.The behaviors of rats(ear redness duration and numbers of head scratching and cage climbing) were observed.The levels of calcitonin gene-related peptide(CGRP)，5-hydroxy tryptamine(5-HT)，and tumor necrosis factor-α(TNF-α) in serum were detected by enzyme linked immunosorbent assay(ELISA).Results:Ear redness appeared about 4 min after nitroglycerin injection and disappear after about 3 h in modeled，LR 3 and non-acupoint groups.Ear redness duration was shorter in LR 3 group compared with that in model and non-acupoint groups(both P<0.05).The numbers of head scratching and cage climbing of rats in model，LR 3 and non-acupoint groups were increased，but there was no significant difference between the groups before acupuncture(P>0.05).After acupuncture of 1 h and 2 h，the numbers of head scratching and cage climbing of rats in LR 3 group were reduced compared with those in model and non-acupoint groups(both P<0.01).Compared with the conditions in blank control group，the serum CGRP and TNF-α were up-regulated(P<0.05)，while 5-HT down-regulated(P<0.05) in the model group.LR 3 group group had lower serum CGRP and TNF-α(both P<0.05) and higher 5-HT(both P<0.05) than those in model and non-acupoint groups.Conclusion:Acupuncture at bilateral Taichong can significantly relieve migraine，and its mechanism may be related to the down-regulation of CGRP and TNF-α as well as the up-regulation of 5-HT in serum. |
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