| 引用本文:马璇1，郝梦2，张明惠3，秦华夏3，杨青青2，李柯翱4，聂继红5,6.没食子软膏对二硝基氯苯致大鼠湿疹的保护作用及机制研究[J].世界中医药,2023,(18):. |
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| 没食子软膏对二硝基氯苯致大鼠湿疹的保护作用及机制研究 |
| Protective Effect and Mechanism of Turkish Gall Ointment on 2,4-Dinitrochlorobenzene-induced Eczema in Rats |
| 投稿时间：2022-01-19 |
| DOI：10.3969/j.issn.1673-7202.2023.18.006 |
| 中文关键词: 没食子软膏 湿疹 苏木精-伊红染色 血液学 免疫组织化学法 荧光定量PCR JAK1/STAT6/PI3K/AKT信号通路 机制研究 |
| English Keywords:Turkish gall ointment Eczema HE staining Hematology Immunohistochemistry Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) JAK1/STAT6/PI3K/AKT signaling pathway Mechanism study |
| 基金项目:新疆维吾尔自治区重点研发计划项目（2018B03018-2）——国内临床缺乏和急需系列儿童药物的研制与开发 |
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| 中文摘要: |
| 目的：分析没食子软膏治疗大鼠湿疹可能的作用机制。方法：选取健康SD大鼠60只，按随机数字表法分为正常组、模型组、阳性组（地塞米松）、没食子软膏低剂量组、没食子软膏中剂量组、没食子软膏高剂量组，除正常组外，其余组均利用7%二硝基氯苯丙酮溶液涂抹背颈部皮肤构建湿疹大鼠模型，模型建立成功后各组连续给予相应药物14 d，取样。试剂盒检测大鼠血液学；苏木精-伊红（HE）染色观察湿疹大鼠病灶处皮肤组织的病理改变；免疫组织化学法测定皮肤中白细胞介素-4受体α、白细胞介素-5、JAK1、p-信号转导及转录激活因子6蛋白表达水平；荧光定量PCR（qRT-PCR）测定皮肤中PI3K、AKT基因表达水平。结果：没食子软膏低剂量组、没食子软膏高剂量组可以显著降低湿疹大鼠血液中单核细胞百分比，没食子软膏中剂量组、没食子软膏高剂量组可以显著降低湿疹大鼠血液中嗜酸性粒细胞百分比；没食子软膏可以显著改善湿疹大鼠的皮肤病理特征，下调皮肤中白细胞介素-4受体α、白细胞介素-5、JAK1、p-STAT6蛋白表达水平，PI3K、AKT基因表达水平。讨论：没食子软膏能够起到治疗湿疹大鼠的作用，其机制可能与影响JAK1/STAT6/PI3K/AKT信号通路有关。 |
| English Summary: |
| To analyze the possible mechanism of Turkish gall ointment in treating eczema in rats.Methods:A total of 60 healthy SD rats were divided into normal group，model group，positive group(dexamethasone)，and low-，medium-，and high-dose Turkish gall ointment groups by random number table.Except for the normal group，all other groups were treated with 7% 2,4-dinitrochlorobenzene(DNCB) and acetone solution by rubbing on the back and neck skin to establish an eczema rat model.After the model was successfully established，each group was given corresponding drugs for 14 consecutive days and samples were collected.Rat hematology was conducted by the kits.HE staining was used to observe the pathological changes of the skin tissues in the lesions of eczema rats.The expressions of IL-4Rα，IL-5，JAK1，and p-STAT6 in the skin were determined by immunohistochemistry(IHC).Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) was performed to detect the expressions of PI3K and AKT genes in the skin.Results:The low-and high-dose Turkish gall ointment significantly reduced the MONO% in the blood of eczema rats，and the medium and high dose significantly reduced the EOS%.Turkish galls ointment improved the pathological characteristics of eczema rat skin，down-regulated the expressions of IL-4Rα，IL-5，JAK1，and p-STAT6 protein as well as PI3K and AKT genes in the skin.Conclusion:Turkish gall ointment is effective in treating eczema，and its mechanism may be related to affecting the JAK1/STAT6/PI3K/AKT signaling pathway. |
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