To analyze the mechanism of Jianpi Xiaopi Prescription combined with massage on gastrointestinal motility and autophagy of interstitial cells of Cajal in anorexia infant rats based on MEK-ERK1/2 signaling pathway.Methods:A total of 50 SPF healthy SD infant rats were randomly divided into healthy group，model group(infantile anorexia model)，traditional Chinese medicine group(model+Jianpi Xiaopi Prescription intervention)，and combined group(model+Jianpi Xiaopi Prescription+massage intervention)，with 10 in each group.Another 10 rats of infantile anorexia model were injected with MEK/ERK signaling pathway inhibitor PD98059，which were used as the inhibitor group.The body weight and food intake of the rats were measured every 2 weeks.Serum amylase was measured by colorimetric method，and serum gastrin was measured by radioimmunoassay.HE staining was used to observe the pathological morphology.The mRNA expressions of c-kit，LC3 and Beclin1 in interstitial cells of Cajal were detected by RT-PCR.The protein expressions of MEK，ERK1/2，P-MEK and P-ERK1/2 were detected by western blot.Results:Compared with the conditions in healthy group，the body weight，growth rate of body weight，food intake，c-kit，intestinal propulsive ratio，serum amylase and serum gastrin were decreased in model group，while MEK，ERK1/2，LC3，Beclin1 and gastric residual volume were increased(P<0.05).After intervention，the body weight，growth rate of body weight，food intake，c-kit，intestinal propulsive ratio，serum amylase and serum gastrin were increased，while MEK，ERK1/2，LC3，Beclin1 and gastric residual volume were decreased(P<0.05)，with the effect of combined group better than that of combined group(P<0.05).There was no significant difference in the protein expression between combined group and inhibitor group(P>0.05).Conclusion:Jianpi Xiaopi Prescription combined with massage may inhibit the autophagy of interstitial cells of Cajal in gastric antrum and improve gastrointestinal function by inhibiting the activation of MEK-ERK1/2 signaling pathway.