| 引用本文:池华博文1，李青松1，2，贾琳琳1，符之瑄1，许愿1，杨倩1，杨俊倚1.推拿促进房水内源性前列腺素的生成调节大鼠睫状体淋巴管增生[J].世界中医药,2023,(22):. |
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| 推拿促进房水内源性前列腺素的生成调节大鼠睫状体淋巴管增生 |
| Massage Regulates Lymphatic Hyperplasia in Ciliary Body of Rats by Promoting Generation of Aqueous Humor Prostaglandin |
| 投稿时间：2022-04-09 |
| DOI：10.3969/j.issn.1673-7202.2023.22.005 |
| 中文关键词: 推拿 房水 前列腺素 拉坦前列腺素 眼压：睫状体 淋巴管 青光眼 |
| English Keywords:Massage Aqueous humor Prostaglandin Latanoprost Intraocular pressure Ciliary body Lymphatic vessels Glaucoma |
| 基金项目:国家自然科学基金项目（82074495）；上海市医学重点专科-眼科建设项目（A-263）；上海市普陀区卫生自主创新科研基金项目(17-PT-07)；上海市普陀区自主创新（重大）项目(15-PT-01)；2020年卫健委临床研究课题（202040148） |
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| 中文摘要: |
| 目的：探讨推拿促进大鼠眼内前列腺素的生成，诱导大鼠眼部睫状体内淋巴管的增生，进而降低青光眼眼压的作用机制。方法：将48只SD大鼠造模后随机分为青光眼模型组、推拿组、拉坦前列腺素组和推拿结合拉坦前列腺素组，每组12例。治疗过程中分别抽取各组不同时间段房水进行酶联免疫吸附试验（ELISA），采用蛋白质印迹法、定量聚合酶链反应、免疫荧光法、免疫组织化学法检测同源盒基因转录因子1（Prox-1）、5'-核苷酸酶（5'-Nase）表达水平，淋巴管酶组织化学染色法检测各组睫状体内淋巴管的增生情况。结果：推拿结合拉坦前列腺素组眼压均低于其他组（P<0.05）；推拿结合拉坦前列腺素组中Prox-1、5'-Nase表达水平均高于其他组（P<0.05）；推拿结合拉坦前列腺素组睫状体组织淋巴增殖均高于其他组。结论：推拿可以有效促进睫状体淋巴管的增生，而增生的淋巴管能吸收多余的房水导致眼压下降。 |
| English Summary: |
| To explore the mechanism of massage in reducing intraocular pressure in glaucoma by promoting the generation of aqueous humor prostaglandin and inducing the intraocular lymphatic hyperplasia in rats.Methods:Forty-eight SD rats were randomized into glaucoma model，massage，latanoprost，and massage+latanoprost groups(n=12).The aqueous humor samples were collected from each group at different time points during the treatment period for enzyme-linked immunosorbent assay.Western blotting，qPCR，immunofluorescence assay，and immunohistochemical staining were employed to determine the expression levels of prospero homeobox 1(Prox-1) and 5'-Nase.Histochemical staining was employ to detect the lymphatic hyperplasia in the ciliary body in each group.Results:The massage+latanoprost group had lower intraocular pressure(P<0.05)，higher expression levels of Prox-1 and 5'-Nase(P<0.05)，and higher rate of lymphatic hyperplasia in the ciliary body than other groups.Conclusion:Massage can promote the lymphatic hyperplasia in the ciliary body，which can facilitate the absorption of excess aqueous humor to reduce intraocular pressure. |
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