To determine the effect of Neiyi Zhitong Decoction on the diagnosis and treatment of endometriosis(EMs) and objectively evaluate its mechanism.Methods:The EMS model was established by autotransplantation，and 40 EM rats were divided into four groups according to the random number table method.A sham operation group was set up with eight rats.Different concentrations of traditional Chinese medicine 985.3 mg/(kg·d) and 245.8 mg/(kg·d) were administered by gavage，and danazol 7.2 mg/(kg·d) was administered by gavage.The model group and sham operation group were given normal saline of 2 mL/d by gavage.After four weeks of treatment，the growth of ectopic lesions was observed，and the volume of ectopic lesions before and after treatment was measured.The indexes were detected by ELISA and Western Blotting.Results:The volume of ectopic lesions in each treatment group decreased significantly(P<0.01)，but there was no significant change in the model group(P>0.05).Compared with the model group，the expression levels of MMP-9，TIMP-1，and E-cadherin in the serum of the Neiyi Zhitong Decoction group were significantly decreased，and the high-dose group was better than the low-dose group(P<0.05).In the tissue homogenate，the expression levels of MMP-9，VEGF，β-catenin，and E-cadherin in the Neiyi Zhitong Decoction group were decreased，and the high-dose group was better than the low-dose group(P<0.05).Conclusion:Neiyi Zhitong Decoction can effectively inhibit the growth of ectopic endometrium，reduce the volume of ectopic lesions，shut down the Wnt/β-catenin signaling pathway by regulating the key regulatory proteins and downstream target genes，and block the occurrence and progression of EMs.