| 引用本文:朱丽1，郑丽文1，王广梅1，李运曼1，蔡宝昌1，李文婷2，毛旭华1.竹节参皂苷调控核因子κB信号通路对肺癌大鼠的影响[J].世界中医药,2024,(02):. |
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| 竹节参皂苷调控核因子κB信号通路对肺癌大鼠的影响 |
| Effect of Panax japonicum Saponin on Lung Cancer Rats by Regulating Nuclear Factor-κB Signaling Pathway |
| 投稿时间：2022-04-16 |
| DOI：10.3969/j.issn.1673-7202.2024.02.005 |
| 中文关键词: 竹节参皂苷 核因子κB 信号通路 肺癌 大鼠 脑神经损伤 微血管密度 肿瘤坏死因子-β |
| English Keywords:Panax japonicus saponins NF-κB Signaling pathway Lung cancer Rats Brain nerve injury Microvascular density TNF-β |
| 基金项目:国家自然科学基金项目（81902148）；江苏省科技成果转化专项资金项目（BA2018002）；江苏省省自然科学基金项目（ZKX17047） |
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| 中文摘要: |
| 目的：探究竹节参皂苷调控核因子κB（NF-κB）信号通路对肺癌大鼠脑神经损伤、微血管密度（MVD）及肿瘤坏死因子-β（TNF-β）表达的影响。方法：选取40只无特定病原体（SPF）级斯泼累格·多雷(SD)雄性大鼠，随机分为正常组、模型组、白术多糖组、竹节参皂苷组，每组10只，对模型组、白术多糖组、竹节参皂苷组采用肺叶支气管内灌注致癌碘化油进行肺癌建模，建模成功后，对白术多糖组灌胃80 mg/kg的白术多糖，对竹节参皂苷组灌胃40 mg/kg的竹节参皂苷，正常组不建模，正常组、模型组同期给予灌胃同体积生理盐水。采用苏木精-伊红（HE）染色法检测肺组织病理形态，TUNEL法检测大鼠脑神经损伤，ELISA法检测大鼠血清中TNF-β水平，免疫组织化学法检测肺组织MVD，蛋白质免疫印迹法检测大鼠肺组织核因子κB蛋白表达。结果：正常组肺组织结构、肺泡正常完整，模型组可见明显病理改变，与模型组比较，白术多糖组、竹节参皂苷组病理结构明显改善，且竹节参皂苷组比白术多糖组改善明显；与正常组比较，模型组海马组织细胞凋亡、MVD、核因子κB蛋白表达明显增多（P<0.05），血清中TNF-β表达明显降低（P<0.05），与模型组比较，白术多糖组、竹节参皂苷组大鼠海马组织细胞凋亡、MVD、核因子κB蛋白表达、肿瘤体积明显降低（P<0.05），血清中TNF-β表达明显升高（P<0.05），且竹节参皂苷组比白术多糖组变化显著（P<0.05）。结论：竹节参皂苷可能是通过负向调控NF-κB信号通路显著改善大鼠脑神经损伤及MVD，提高TNF-β表达水平。 |
| English Summary: |
| To investigate the effect of Panax japonicus saponin on brain nerve injury，microvascular density(MVD) and TNF-1β in lung cancer rats by regulation of NF-κB signaling pathway.Methods:Forty SPF male SD rats were randomly divided into normal group(A)，model group(B)，Atractylodes atractylodes polysaccharide(C) group and P.japonicus saponin(D) group，with 10 in each group.Lung cancer modeling was performed in groups B，C and D by endobronchial perfusion of carcinogenic iodide oil.After successful modelling，group C was intragastrically administered with 80 mg/kg A.atractylodes polysaccharide; group D was intragastrically administered with 40 mg/kg P.japonicus saponin; groups A and B were given the same volume of normal saline by gavage.HE staining was used to observe the pathological morphology of lung tissue，and TUNEL assay was performed to detect brain nerve injury.ELISA was performed to detect the serum TNF-β of rats.MVD in lung tissue was determined by immunohistochemistry，and NF-κB protein expression in lung tissue was measured by Western blot.Results:Lung tissue and alveoli were normal and intact in group A，while obvious pathological changes were seen in group B.Compared with the condition in group B，the pathological structure in groups C and D was significantly improved，and group D was better than group C.Compared with group A，group B had increased hippocampal cell apoptosis，MVD，and NF-κB protein expression(P<0.05)，and decreased serum TNF-β(P<0.05).Hippocampal cell apoptosis，MVD，NF-κB protein expression and tumor volume in groups C and D were lower than those in group B(P<0.05)，while the serum TNF-β was higher(P<0.05)，with group D having more obvious changes than group C(P<0.05).Conclusion:P.japonicus saponins may significantly improve brain nerve injury and MVD by negatively regulating NF-κB signaling pathway and increasing TNF-β in rats. |
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