| 引用本文:王沁筠1，2，3，王晓赟4，袁璐莹1，2，徐浩1，2，5，施杞1，2，5，梁倩倩1，2，5，李宁1，2，5，王拥军1，2，5.热痹方对痛风性关节炎小鼠炎症标志物表达的影响[J].世界中医药,2024,(02):. |
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| 热痹方对痛风性关节炎小鼠炎症标志物表达的影响 |
| Effects of Rebi Formula on the Expression of Inflammatory Markers in Gouty Arthritis Mice |
| 投稿时间：2023-01-28 |
| DOI：10.3969/j.issn.1673-7202.2024.02.006 |
| 中文关键词: 中医药 热痹方 痛风性关节炎 小鼠 炎症介质 白细胞介素-1β 白细胞介素-6 肿瘤坏死因子-α |
| English Keywords:Traditional Chinese medicine Rebi formula Gouty arthritis Mouse Inflammatory cytokines Interleukin-1β Interleukin-6 Tumor necrosis factor-α |
| 基金项目:教育部创新团队项目（IRT1270）；上海市卫生健康委员会中医药科研项目创新团队类项目（2022CX001）；国家中医药管理局-青年岐黄学者项目（2015RA4002）；上海市重中之重研究中心（2022ZZ01009）；中国博士后基金面上项目（2022M722163） |
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| 中文摘要: |
| 目的：研究热痹方对痛风性关节炎小鼠的治疗作用及对炎症介质白细胞介素-1β（IL-1β）、IL-6及肿瘤坏死因子-α（TNF-α）的抑制作用。方法：30只C57BL/6小鼠随机分为对照组、模型组、低剂量热痹方组、高剂量热痹方组、秋水仙碱组。除对照组外，其余4组小鼠均在足底关节附近注射3%尿酸钠晶体悬液制备痛风性关节炎（GA）小鼠模型。造模后6 h，对照组和模型组小鼠灌胃给予纯净水，低、高剂量热痹方组小鼠分别灌胃给予热痹方提取物（17.82 g/kg，35.64 g/kg），秋水仙碱组小鼠腹腔注射给予秋水仙碱溶液（0.5 mg/kg）。观察治疗前后小鼠体质量、足肿胀、热痛阈的变化；苏木精-伊红染色评估足组织、肝脏和肾脏炎症变化；采用酶联免疫吸附试验（ELISA）法检测足组织中IL-1β、IL-6、TNF-α的含量；实时PCR（RT-PCR）检测足组织中IL-1β、IL-6、TNF-α的基因表达水平。结果：与模型组比较，高剂量热痹方组或秋水仙碱组能显著减轻GA小鼠后爪肿胀程度（P<0.01），提高热痛耐受性（P<0.001），减少关节组织的炎症细胞浸润，且明显降低关节组织炎症介质IL-1β、IL-6、TNF-α的蛋白和mRNA水平（P<0.001）。另外，高剂量热痹方和秋水仙碱对GA小鼠治疗效果无明显差异（P>0.05）。结论：热痹方能有效改善GA小鼠症状并抑制关节炎症反应，且对肝肾功能无明显影响。 |
| English Summary: |
| To investigate the therapeutic effect of Rebi formula on mice with gouty arthritis(GA) and the inhibition effects on inflammation factors interleukin-1β(IL-1β)，interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α).Methods:Thirty C57BL/6 mice were randomly divided into control group，model group，low-dose Rebi formula group，high-dose Rebi formula group and colchicine group.Except for the control group，mice in the other four groups were injected with 3% monosodium urate crystal suspension near the plantar joint to generate mouse model of GA.Six hours after modeling，mice in the control group and the model group were given distilled water by intragastric administration.The mice in the low-dose and high-dose Rebi formula groups were given Rebi formula extracts(17.82 g/kg，35.64 g/kg) by gavage，and mice in the colchicine group were given colchicine solution intraperitoneally(0.5 mg/kg).The changes in body weight，foot swelling and thermal pain threshold were observed before and after the treatment.Hematoxylin-eosin staining was used to evaluate the changes of inflammation in foot tissue，liver and kidney.The contents of IL-1β，IL-6 and TNF-α in foot tissue were determined by ELISA.Gene expression levels of IL-1β，IL-6 and TNF-α in foot tissues were detected by qRT-PCR.Results:Compared with model group，high-dose Rebi formula or colchicine treatment significantly reduced the swelling degree of the hind paw of GA mice(P<0.01)，improved the heat pain tolerance(P<0.001)，and reduced the inflammatory cell infiltration of joint tissue.The protein and mRNA expression levels of inflammatory factors IL-1β，IL-6 and TNF-α were significantly decreased in joint tissue(P<0.001).In addition，there was no significant difference in the therapeutic effect between high-dose Rebi formula group and colchicine group(P>0.05).Conclusion:Rebi formula can effectively alleviate the symptoms and inhibit joint inflammation in mice with GA，with no significant effect on liver and kidney function. |
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