| 引用本文:补娟1，史深2，叶勒丹·马汉1，王兆霞1，周玲1.刺槐素通过核因子κB信号通路抑制脂多糖诱导的巨噬细胞损伤[J].世界中医药,2024,(03):. |
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| 刺槐素通过核因子κB信号通路抑制脂多糖诱导的巨噬细胞损伤 |
| Inhibition of LPS-induced Macrophage Injury by Acacetin via Regulating NF-κB Signaling Pathway |
| 投稿时间：2022-08-16 |
| DOI：10.3969/j.issn.1673-7202.2024.03.004 |
| 中文关键词: 刺槐素 脂多糖 核因子κB 巨噬细胞 抑制 抗炎作用 磷酸化 核因子κB抑制蛋白α |
| English Keywords:Acacetin LPS NF-κB Macrophages Inhibition Anti-inflammatory effects Phosphorylation IκBα |
| 基金项目:新疆维吾尔自治区天山青年计划项目（2020Q046）；乌鲁木齐市优秀青年科技人才培养项目 |
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| 中文摘要: |
| 目的：探讨刺槐素（Acacetin）对脂多糖（LPS）诱导的小鼠骨髓巨噬细胞（BMDMs）的抗炎作用机制。方法：CCK-8法检测各浓度刺槐素（5 μmol/L，10 μmol/L，20 μmol/L，40 μmol/L）对小鼠骨髓巨噬细胞增殖的影响，筛选最佳剂量；分离培养小鼠骨髓巨噬细胞，LPS（50 ng/mL）预处理BMDMs（0 min，10 min，30 min，60 min），加入刺槐素（10 μmol/L）孵育0.5 h。蛋白质印迹法检测p65、磷酸化p65（p-p65）、核因子κB抑制蛋白α（IκBα）、磷酸化IκBα（p-IκBα）的表达，激光共聚焦观察核因子κB核转位情况。结果：CCK-8结果显示，5~40 μmol/L的刺槐素对小鼠骨髓巨噬细胞增殖无明显影响，结合课题组前期研究结果，选择10 μmol/L刺槐素作为后续研究剂量；与空白对照组比较，LPS不同时间刺激组p-p65、p-IκBα的表达水平均显著增加，给予刺槐素治疗后，能显著降低p-核因子κB p65和p-IκBα的表达；刺槐素能抑制LPS介导的核因子κB p65核转位。结论：刺槐素的抗炎作用与抑制核因子κB信号通路有关，刺槐素可作为一种有潜力的候选抗炎药物。 |
| English Summary: |
| To explore the anti-inflammatory mechanisms of acacetin in lipopolysaccharide(LPS)-induced mouse bone marrow-derived macrophages(BMDMs).Methods:The effect of various concentrations of acacetin(5，10，20，and 40 μmol/L) on the proliferation of BMDMs was tested using the CCK-8 method，so as to select the optimal dosage.BMDMs were isolated and cultured，and LPS(50 ng/mL) was used to pretreat BMDMs for 0，10，30，and 60 minutes.Then，BMDMs were added with acacetin(10 μmol/L) and cultured for 0.5 h.Western blotting was used to analyze the expression of phosphorylated p65(p-p65)，p65，nuclear factor(NF) κB suppressor protein α(IκBα)，and phosphorylated IκBα(p-IκBα).The nuclear translocation of NF κB was detected by laser scanning confocal microscope(LSCM).Results:CCK-8 results showed that acacetin concentrations ranging from 5 to 40 μmol/L had no significant effect on the proliferation of BMDMs.Based on previous studies of the team，10 μmol/L of acacetin was chosen for subsequent experiments.Compared with the blank control group，LPS stimulation significantly increased the expression levels of p-p65 and p-IκBα.Treatment with acacetin significantly reduced the expression levels of p-NF-κB p65 and p-IκBα.Moreover，acacetin inhibited the LPS-induced nuclear translocation of NF-κB p65.Conclusion:The anti-inflammatory effects of acacetin were related to the inhibition of the NF-κB signaling pathway，suggesting acacetin may be a potential anti-inflammatory drug. |
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