| 引用本文:李朕1，2，张洪亮3，严胜利2，陈月婵2，邬超4，蔡钢2.紫龙金联合埃克替尼对Lewis肺癌荷瘤小鼠免疫功能及肿瘤血管生成的影响[J].世界中医药,2024,(03):. |
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| 紫龙金联合埃克替尼对Lewis肺癌荷瘤小鼠免疫功能及肿瘤血管生成的影响 |
| Effects of Zilongjin Combined with Icotinib on Immune Function and Tumor Angiogenesis in Lewis Lung Carcinoma Tumor-bearing Mice |
| 投稿时间：2023-09-19 |
| DOI：10.3969/j.issn.1673-7202.2024.03.007 |
| 中文关键词: 肺癌 Lewis肺癌 小鼠 紫龙金 埃克替尼 血管生成 免疫功能 |
| English Keywords:Lung cancer Lewis lung carcinoma Mice Zilongjin Icotinib Angiogenesis Immune function |
| 基金项目:第四届国医大师传承工作室和第二届全国名传承工作室建设项目（国中医药人教函〔2022〕75号） |
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| 中文摘要: |
| 目的：探讨紫龙金联合埃克替尼对Lewis肺癌荷瘤小鼠免疫功能及肿瘤血管生成的影响。方法：选用无特定病原体（SPF）级C57BL/6雄性小鼠50只，随机取10只用于制备Lewis瘤细胞悬液，余40只于其右前肢腋窝皮下分别接种0.2 mL的Lewis瘤细胞悬液，建立Lewis肺癌荷瘤小鼠模型，并将其随机均分为模型组、紫龙金组、埃克替尼组、联合观察组，每组10只。对比各组肿瘤重量、肿瘤生长抑制率；用酶联免疫吸附试验（ELISA）法检测小鼠血管内皮生长因子、小鼠肿瘤组织白细胞介素2（IL-2）、肿瘤坏死因子α(TNF-α)、干扰素(IFN-γ)水平；流式细胞术检测小鼠脾脏免疫细胞比例。结果：与紫龙金组及埃克替尼组比较，联合观察组小鼠的肿瘤重量和小鼠肿瘤生长抑制率高（均P<0.05），血管内皮生长因子水平及微血管密度低（均P<0.05），CD4+T、CD8+T及B淋巴细胞比例高（均P<0.05），肿瘤组织的IL-2、TNF-α、IFN-γ水平低（均P<0.05）。结论：紫龙金和埃克替尼联合治疗具有协同作用，显著降低了Lewis肺癌荷瘤小鼠模型的肿瘤重量，抑制了血管生成，增强了免疫功能，减轻了炎症反应。 |
| English Summary: |
| To investigate the effects of Zilongjin combined with Icotinib on the immune function and tumor angiogenesis in Lewis lung carcinoma(LLC) tumor-bearing mice.Methods:Fifty specific pathogen-free(SPF) male C57BL/6 mice were selected.Ten mice were randomly chosen to prepare LLC cell suspension，and the remaining 40 mice were subcutaneously inoculated with 0.2 mL of LLC cell suspension in the right forelimb axilla to establish the LLC tumor-bearing mouse model.The mice were then randomly divided into the model group，Zilongjin group，Icotinib group，and combination group，with 10 mice in each group.Tumor weight and tumor growth inhibition rate were compared among the groups.Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of vascular endothelial growth factor(VEGF)，interleukin-2(IL-2)，tumor necrosis factor-alpha(TNF-α)，and interferon-gamma(IFN-γ) in mouse serum，and flow cytometry was used to detect the proportion of immune cells in the spleen.Results:Compared with the Zilongjin and Icotinib groups，the combination group showed higher tumor weight and tumor growth inhibition rate in LLC tumor-bearing mice(all P<0.05)，lower levels of VEGF and microvessel density(both P<0.05)，higher proportions of CD4+T，CD8+T，and B lymphocytes(all P<0.05)，and lower levels of IL-2，TNF-α，and IFN-γ in tumor tissues(all P<0.05).Conclusion:Zilongjin combined with Icotinib has a synergistic effect，which can significantly reduce tumor weight in LLC tumor-bearing mice，inhibit angiogenesis，enhance immune function，and alleviate inflammatory reactions. |
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