世界中医药
文章摘要
引用本文:吕树泉1,张辉2,张淑芳1,潘保朝2,姚彬1,苏秀海1,王元松1,李文东1,李函舟3,田甜1,苗静4.白虎加人参汤对2型糖尿病大鼠脂代谢的影响[J].世界中医药,2024,(07):.  
白虎加人参汤对2型糖尿病大鼠脂代谢的影响
Effects of Baihu Decoction with Ginseng on Lipid Metabolism in Type 2 Diabetic Rats
投稿时间:2022-09-14  
DOI:10.3969/j.issn.1673-7202.2024.07.009
中文关键词:  白虎加人参汤  2型糖尿病  血糖  脂代谢  腺苷一磷酸活化蛋白激酶/固醇反应元件结合蛋白-1(AMPK/SREBP1)信号通路  中医药  作用机制  实验研究
English Keywords:Baihu Decoction with Ginseng  Type 2 Diabetes  Blood Glucose  Lipid Metabolism  AMPK/SREBP1 Signaling Pathway  Traditional Chinese Medicine  Mechanism of Action  Experimental Study
基金项目:国家自然科学基金项目(82274424);沧州市重点研发计划项目(213106042);苏秀海全国名老中医药专家传承工作室(国中医药人教函〔2022〕75号)
作者单位
吕树泉1,张辉2,张淑芳1,潘保朝2,姚彬1,苏秀海1,王元松1,李文东1,李函舟3,田甜1,苗静4 1 河北中医学院附属沧州中西医结合医院沧州061001 2 河北中医学院研究生院石家庄050091 3 承德医学院研究生院承德067000 4 天津市第二人民医院天津300192 
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中文摘要:
      目的:研究白虎加人参汤对2型糖尿病(T2DM)模型大鼠腺苷一磷酸活化蛋白激酶(AMPK)/固醇反应元件结合蛋白-1(SREBP1)信号通路的影响。方法:通过高脂饲料联合链脲佐菌素(STZ)注射建立T2DM模型大鼠,并用不同剂量的白虎加人参汤灌胃。通过检测各组大鼠造模给药后血清中相关生化指标和肝组织病理学变化,评估白虎加人参汤对T2DM模型大鼠的治疗作用;然后,运用定量聚合酶链反应(qPCR)和蛋白质免疫分析法检测白虎加人参汤干预后,各组大鼠肝组织中AMPK/SREBP1信号通路关键因子腺苷一磷酸活化蛋白激酶(AMPK)、乙酰辅酶A羧化酶1(ACC1)、脂肪酸合成酶(FASN)、ATP柠檬酸裂解酶(ACLY)基因及蛋白水平,初步探究白虎加人参汤治疗T2DM模型大鼠的作用机制。结果:白虎加人参汤可明显降低T2DM模型大鼠血清中空腹血糖(FBG)、口服葡萄糖耐量试验(OGTT)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)、总胆固醇(TC)、三酰甘油(TG)以及低密度脂蛋白胆固醇(LDL-C)水平,升高高密度脂蛋白胆固醇(HDL-C)水平,同时改善T2DM模型大鼠肝组织病理学变化。qPCR及蛋白质免疫分析结果显示,白虎加人参汤可显著降低T2DM模型大鼠肝组织中AMPK基因与蛋白水平,上调SREBP1、ACC1、ACLY、FASN基因与蛋白水平。结论:白虎加人参汤对T2DM模型大鼠具有治疗作用,其作用机制可能与调节肝组织中AMPK/SREBP1信号通路,改善脂代谢有关。
English Summary:
      To investigate the effect of Baihu Decoction with Ginseng on AMP-activated protein kinase/sterol regulatory element-binding protein-1(AMPK/SREBP1) signaling pathway in type 2 diabetes mellitus(T2DM) model rats.Methods:T2DM model rats were established using high-fat feed combined with streptozotocin(STZ) injection,followed by intragastrically administrating different doses of Baihu Decoction with Ginseng.The therapeutic effect of Baihu Decoction with Ginseng on T2DM model rats was evaluated by detecting the relevant serum biochemical indices and liver histopathological changes after intragastrical administration in each group of rats; Then,after the Baihu Decoction with Ginseng intervention was detected by qPCR and Western blot method,the genes and protein levels of key factors AMPLK/SREBP1 signaling pathway,such as AMPK,acetyl-coenzyme A carboxylase 1(ACC1),fatty acid synthase(FASN),and ATP citrate lyase(ACLY) were detected in the liver tissues of rats in each group,and the mechanism of action of Baihu Decoction with Ginseng in the treatment of T2DM model rats was preliminarily explored.Results:Baihu Decoction with Ginseng significantly reduced the levels of fasting blood glucose(FBG),oral glucose tolerance test(OGTT),glutamate-pyruvate transaminase(GPT),glutamic-oxaloacetic transaminase(GOT),total cholesterol(TC),triglycerides(TG) and low-density lipoprotein cholesterol(LDL-C) in the serum of T2DM models,and increased the levels of high-density lipoprotein cholesterol(HDL-C).It also improved liver histopathological changes in the liver of T2DM model rats.The qPCR and Western blot results showed that Baihu Decoction with Ginseng could significantly reduce the levels of AMPK gene and protein levels in the liver tissue of T2DM model rats,and upregulate the genes and proteins levels of SREBP1,ACC1,ACLY,FASN.Conclusion:Baihu Decoction with Ginseng has a therapeutic effect on T2DM model rats,possibly by modulating the AMPK/SREBP1 signaling pathway in liver tissue and improving lipid metabolism.
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