| 引用本文:白新宇1，孙士伟1，王志鹏1，尹跃伟2.红芪多糖对线粒体途径介导的膀胱癌细胞凋亡的影响[J].世界中医药,2024,(07):. |
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| 红芪多糖对线粒体途径介导的膀胱癌细胞凋亡的影响 |
| Effects of Hedysarum Polysaccharides on Apoptosis of Bladder Cancer Cells Mediated by Mitochondrial Pathway |
| 投稿时间：2022-06-23 |
| DOI：10.3969/j.issn.1673-7202.2024.07.012 |
| 中文关键词: 红芪多糖 线粒体 膀胱癌 凋亡 Bcl-2相关X蛋白 c-Jun氨基末端激酶 半胱氨酸蛋白酶3 |
| English Keywords:Hedysarum polysaccharides Mitochondrion Bladder cancer Apoptosis Bcl-2-related X protein c-Jun N-terminal kinase(JNK) Caspase-3 |
| 基金项目:2020河北省医学科学研究计划项目（20200936） |
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| 中文摘要: |
| 目的：观察红芪多糖对线粒体途径介导的膀胱癌细胞凋亡的影响，并探讨可能机制。方法：取对数期T24细胞，随机分为对照组（常规培养）、红芪多糖组（加入红芪多糖0.8 μg/L）、SP600125组（加入SP600125 10 μmol/L）、联合组（加入红芪多糖0.8 μg/L、SP600125 10 μmol/L）。MTT法检测细胞增殖能力；双染法检测细胞凋亡率；JC-1法检测细胞线粒体损伤；免疫印迹法检测细胞相关蛋白表达。结果：与对照组比较，红芪多糖组24、48、72 h吸光度值、线粒体膜电位、B淋巴细胞瘤-2（Bcl-2）蛋白表达量降低；凋亡率，Bcl-2相关X蛋白（Bax）、半胱氨酸蛋白酶3（Caspase-3）蛋白表达量，p-c-Jun氨基末端激酶（JNK）/JNK、p-c-Jun/c-Jun升高（均P<0.05）。SP600125组24、48、72 h吸光度值，线粒体膜电位、Bcl-2蛋白表达量升高；凋亡率，Bax、Caspase-3蛋白表达量，p-JNK/JNK、p-c-Jun/c-Jun降低（均P<0.05）。与红芪多糖组比较，联合组24、48、72 h吸光度值、线粒体膜电位、Bcl-2蛋白表达量升高；凋亡率，Bax、Caspase-3蛋白表达量，p-JNK/JNK、p-c-Jun/c-Jun降低（均P<0.05）。结论：红芪多糖可抑制膀胱癌T24细胞增殖，并通过介导线粒体途径促进其凋亡，作用机制可能与激活JNK/c-Jun信号通路有关。 |
| English Summary: |
| To observe the effects of Hedysarum polysaccharides(HPS) on the apoptosis of bladder cancer cells mediated by mitochondrial pathway，and to explore the possible mechanism.Methods:T24 cells in log phase were collected and randomly divided into control group(conventional culture)，Hedysarum polysaccharides group(adding Hedysarum polysaccharides 0.8 μg/L)，SP600125 group(adding SP600125 10 μmol/L)，and combination group(adding Hedysarum polysaccharides) 0.8 μg/L，SP600125 10 μmol/L).MTT assay was used to detect cell proliferation.Double staining was used to detect the apoptosis rate.JC-1 method was used to detect mitochondrial damage in cells.Western blotting was used to detect expressions of cell-related proteins.Results:Compared with the control group，the absorbance values at 24，48 and 72 h，mitochondrial membrane potential，Bcl-2 protein expression were decreased，apoptosis rate，expressions of Bax and Caspase-3 proteins，p-JNK/JNK，p-c-Jun/c-Jun were increased in the HPS group(P<0.05)，and the absorbance values at 24，48 and 72 h，mitochondrial membrane potential，Bcl-2 protein expression were increased，apoptosis rate，expressions of Bax and Caspase-3 proteins，p-JNK/JNK，p-c-Jun/c-Jun were decreased in the SP600125 group(P<0.05).Compared with the HPS group，the absorbance values at 24，48 and 72 h，mitochondrial membrane potential，and Bcl-2 protein expression were increased，and apoptosis rate，expressions of Bax and Caspase-3 proteins，p-JNK/JNK，p-c-Jun/c-Jun were decreased in the combination group(P<0.05).Conclusion:HPS can inhibit the proliferation of T24 cells and promote their apoptosis by mediating the mitochondrial pathway.The mechanism may be related to the activation of the JNK/c-Jun signaling pathway. |
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