To explore the effects of Jianpi Wenshen Formula(JPWSF) on the levels of immune cell-related factors in the ascites model of sarcoma-180(S180)-bearing mice.Methods:Sixty C57 mice of 5 to 6 weeks old were randomized into a model group，a cisplatin group，a cisplatin+low-dose(9.75 g/kg) JPWSF group，and a cisplatin+high-dose(19.5 g/kg) JPWSF group，with 15 mice in each group.A ascites model of S180-bearing mice was established.The mice were administrated with cisplatin at a dose of 0.2 mg/kg by gavage on days 1，4，and 7 of each week for four weeks.JPWSF was administrated at corresponding doses once a day for a total of 4 weeks.The model group received intraperitoneal injection of an equal dose of normal saline on days 1，4，and 7 of each week for 4 weeks.The tumor weight，tumor inhibition rate，ascites liquid volume，red blood cell and tumor cell counts，carcino-embryonic antigen(CEA) level，and levels of immune cell-related factor were compared between groups.The survival time of the mice was recorded.Results:Compared with the cisplatin group，the additional administration of JPWSF increased the tumor inhibition rate in a dose-dependent manner(P<0.05).Compared with the model group，the cisplatin，cisplatin+low-dose JPWSF，and cisplatin+high-dose JPWSF groups showed decreased tumor weight，ascites liquid volume，red blood cell count，and tumor cell count，lowered levels of CEA，vascular endothelial growth factor(VEGF)，and interleukin-4(IL-4)，elevated level of interferon-gamma(IFN-γ)，and prolonged the survival time(P<0.05).Compared with the cisplatin group，the additional administration of JPWSF reduced the tumor weight，ascites liquid volume，red blood cell count，and tumor cell count，lowered the levels of CEA，VEGF，and IL-4，elevated the IFN-γ level，and prolonged the survival time in a dose-dependent manner(P<0.05).Conclusion:JPWSF significantly inhibited the tumor，reduced the ascites liquid，lowered the VEGF and IL-4 levels，and elevated the IFN-γ level in the ascites model of S180-bearing mice.