| 引用本文:王心威1，2，李军梅2，彭勍2，朱萱萱2，刘建勋1，2.6-姜烯酚对急性肺损伤小鼠肺水肿的治疗作用及机制研究[J].世界中医药,2024,(08):. |
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| 6-姜烯酚对急性肺损伤小鼠肺水肿的治疗作用及机制研究 |
| Therapeutic Effect and Mechanism of 6-shogaol on Pulmonary Oedema in the Mouse Model of Acute Lung Injury |
| 投稿时间：2023-10-17 |
| DOI：10.3969/j.issn.1673-7202.2024.08.004 |
| 中文关键词: 6-姜烯酚 脂多糖 急性肺损伤 炎症反应 肺水肿 Toll样受体4/核因子κB/水通道蛋白通路 水通道蛋白1 水通道蛋白5 |
| English Keywords:6-Shogaol Lipopolysaccharide Acute lung injury Inflammation Pulmonary edema Toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)/aquaporin(AQP) pathway Aquaporin 1 Aquaporin 5 |
| 基金项目:国家自然科学基金重点资助项目(82030124)；中国中医科学院科技创新工程项目(CI2021A04609) |
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| 中文摘要: |
| 目的：观察6-姜烯酚（6-SH）对脂多糖（LPS）诱导的急性肺损伤（ALI）小鼠肺水肿的治疗作用，探究可能的作用机制。方法：采用随机数字表法将40只无特定病原体（SPF）级Balb/c小鼠分为对照组、模型组、6-姜烯酚组、地塞米松组，每组10只。鼻腔滴注LPS构建ALI模型，对照组滴注等量生理盐水。30 min后，给予6-姜烯酚组灌胃（100 mg/kg）；地塞米松组给予腹腔注射（5 mg/kg）；对照组和模型组给予灌胃等量生理盐水，24 h后结束实验，收集样本。测定肺组织湿/干重比（W/D）和肺系数；收集支气管肺泡灌洗液（BALF），测定白细胞计数和总蛋白浓度；观察肺组织病理学改变、计算病理评分；测定BALF上清液中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）以及白细胞介素-6（IL-6）水平；检测肺组织中Toll样受体4/核因子κB/水通道蛋白（TLR4/NF-κB/AQPs）信号通路相关蛋白表达水平。结果：与对照组比较，模型组肺组织损伤明显，病理评分、肺系数、W/D增加（P<0.01），白细胞计数、总蛋白浓度及TNF-α、IL-1β、IL-6的水平升高（P<0.01）；与模型组比较，6-姜烯酚组的肺系数和W/D降低（P<0.01或P<0.05），白细胞计数、总蛋白浓度以及TNF-α、IL-1β、IL-6的水平降低（P<0.01或P<0.05），AQP1和AQP5的蛋白表达水平升高（P<0.01），TLR4、MyD88、NF-κB p65以及p-NF-κB p65的表达水平降低（P<0.01或P<0.05）。结论：6-姜烯酚可能通过调控TLR4/NF-κB/AQPs信号通路发挥其抗炎作用和改善肺组织水液代谢与转运，有效缓解ALI小鼠的肺组织水肿。 |
| English Summary: |
| To observe the therapeutic effect of 6-shogaol(6-SH) on lipopolysaccharide(LPS)-induced pulmonary edema in the mouse model of acute lung injury(ALI) and investigate the possible mechanism.Methods:Forty specific pathogen free(SPF)-grade Balb/c mice were assigned into control，model，6-SH(100 mg/kg)，and dexamethasone(5 mg/kg) groups by the random number table method，with 10 mice in each group.The mouse model of ALI was established by nasal dripping of LPS，and the control group was dripped with an equal amount of normal saline.Thirty minutes after modeling，mice in corresponding groups were administrated with 6-SH by gavage and dexamethasone by intraperitoneal injection.The control and model groups were administrated with an equal amount of normal saline by gavage.The experiment was ended and samples were collected 24 h later.The wet/dry weight(W/D) ratio of the lung tissue and the lung index were determined.The bronchoalveolar lavage fluid(BALF) was collected to determine the white blood cell count and total protein concentration.Hematoxylin-eosin(HE) staining was employed to observe the pathological changes in the lung tissue，and the pathological scores were calculated.Enzyme-linked immunosorbent assay was employed to determine the levels of tumor necrosis factor-α(TNF-α)，interleukin-1β(IL-1β)，and IL-6 in the supernatant of BALF.Western blotting was employed to determine the expression levels of proteins related to the Toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)/aquaporin(AQP) signaling pathway in the lung tissue.Results:Compared with the control group，the model group showed obvious lung injury，increased pathological scores，lung index，W/D ratio，leukocyte count，total protein concentration，and levels of TNF-α，IL-1β，and IL-6(P<0.01).Compared with the model group，6-SH decreased the W/D ratio，lung index，leukocyte count，total protein concentration，and levels of TNF-α，IL-1β，and IL-6，up-regulated expression levels of AQP1 and AQP5(P<0.01)，and down-regulated expression levels of TLR4，MyD88，NF-κB p65，and p-NF-κB p65(P<0.01 or P<0.05).Conclusion:6-SH may exert the anti-inflammatory effect and improve the water-liquid metabolism and transport in the lung tissue by modulating the TLR4/NF-κB/AQP signaling pathway to alleviate the pulmonary edema in ALI mice. |
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