| 引用本文:赵帅1，连方2.经皮穴位电刺激对多囊卵巢综合征卵母细胞的影响[J].世界中医药,2024,(08):. |
|
| 经皮穴位电刺激对多囊卵巢综合征卵母细胞的影响 |
| Effects of Percutaneous Acupoint Electrical Stimulation on Oocyte Quality in Patients with Polycystic Ovary Syndrome |
| 投稿时间：2023-02-10 |
| DOI：10.3969/j.issn.1673-7202.2024.08.017 |
| 中文关键词: 经皮穴位电刺激 痰湿型 多囊卵巢综合征 卵泡刺激激素受体 卵细胞质量 基因组学 |
| English Keywords:Percutaneous acupoint electrical stimulation Syndrome of phlegm dampness Polycystic ovarian syndrome Follicle-stimulating hormone receptor Oocyte quality Genomics |
| 基金项目:国家自然科学基金项目（82104914）——基于卵泡液-颗粒细胞代谢路径探讨燥湿化痰中药对痰湿型PCOS卵母细胞发育潜能及能量代谢的影响；山东省自然科学基金项目(制ZR2020QH313)——基于NF-KappaB/Jak-STAT/SOC信号通路探讨苍附导痰汤改善PCOS大鼠卵子及胚胎质量的相关机制 |
|
| 摘要点击次数: 362 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：通过经皮穴位电刺激对多囊卵巢综合征（PCOS）卵母细胞及卵泡刺激素受体（FSHR）的影响，探究其作用机制。方法：选取2020年10月至2021年9月就诊于山东中医药大学妇产生殖中心符合痰湿型PCOS患者作为研究对象，采用随机数字表法分为观察组（经皮穴位电刺激联合西药）和对照组（伪针联合西药），每组50例，另选取因男方因素行IVF的正常组（西药）患者50例。分别观察PCOS患者治疗前后证候积分及积分差值、3组患者促性腺激素(Gn)总量、取卵数、单卵雌激素水平、优卵率、受精率、优质胚胎率、临床妊娠率，取卵日卵巢颗粒细胞亲吻素/亲吻素受体1（Kiss1R）系统中FSHR水平的表达差异。结果：观察组与对照组比较，治疗前后证候积分、积分差值改善（P<0.05）；Gn总量、优胚率、临床妊娠率比较，差异无统计学意义(P>0.05)；3组单卵雌激素水平、获卵数、优卵率、受精率显著差异（P<0.05）；观察组患者颗粒细胞FSHR的表达水平较对照组有明显上调，差异有统计学意义（P<0.05）。结论：经皮穴位电刺激提高PCOS卵母细胞的作用与上调通路中关键因子FSHR的表达，改善微环境并促进细胞增殖有关。 |
| English Summary: |
| To explore the effect and mechanism of percutaneous acupoint electrical stimulation on the oocyte quality and follicle-stimulating hormone receptor(FSHR) in the patients with polycystic ovary syndrome(PCOS).Methods:The PCOS patients with the syndrome of phlegm dampness treated in the Obstetrics and Gynecology Center of Shandong University of Traditional Chinese Medicine from October 2020 to September 2021 were enrolled in this study.The patients were assigned by the random number table method into an observation group(percutaneous acupoint electrical stimulation combined with Western medicine) and control group(sham acupuncture combined with Western medicine)，with 50 patients in each group.In addition，50 patients who underwent in-vitro fertilization(IVF) due to male reasons were selected as the normal group(Western medicine).The scores of TCM symptoms，differences in the scores，total level of gonadotropin(Gn)，oocyte retrieval number，level of single oocyte estrogen，high-quality oocyte rate，fertilization rate，high-quality embryo rate，clinical pregnancy rate，and the expression of FSHR in the Kiss1R system of ovarian granulosa cells on the day of oocyte retrieval were determined before and after treatment in the three groups.Results:Compared with the control group，the observation group showed improvement in terms of the TCM symptom scores and score differences(P<0.05)，no significant differences in total Gn，high-quality embryo rate，or clinical pregnancy rate(P>0.05)，and differences in the levels of single oocyte estrogen，oocyte retrieval number，high-quality oocyte rate，and fertilization rate(P<0.05).In addition，the expression level of FSHR in granulosa cells in the observation group was up-regulated compared with that in the control group(P<0.05).Conclusion:Percutaneous acupoint electrical stimulation may improve the oocyte quality by up-regulating the expression of FSHR and the microenvironment and promote the cell proliferation in the PCOS patients. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
