To study the relationship between the high performance liquid chromatography(HPLC) fingerprint and the phlegm-dispelling effect of Farfarae Flos extract，and clarify the bioactive components of Farfarae Flos for dispelling phlegm.Methods:HPLC was employed to establish the fingerprint of Farfarae Flos extract and determine the content of chemical components.The phlegm-dispelling effect of Farfarae Flos extract was evaluated based on the volume of phenol red in mouse trachea.The spectrum-effect relationship was analyzed by partial least squares regression.Results:The bioactive components in Farfarae Flos extract for dispelling phlegm included rutin，isoquercitrin，chlorogenic acid，neochlorogenic acid，cryptochlorogenic acid，isochlorogenic acid A，isochlorogenic acid B，isochlorogenic acid C，and the components corresponding to peaks 3 and 17.Conclusion:The phlegm-dispelling effect of Farfarae Flos was the result of the joint actions of multiple components.This study provides a reference for establishing an effect-related quality standard of Farfarae Flos.