世界中医药
文章摘要
引用本文:刘洪波,靖春颖,黎正卿,刘彩凤,张熙思,蒲新伟,林日亮.基于Wnt/β-catenin信号通路的健骨伸筋汤对膝骨关节炎患者的干预作用研究[J].世界中医药,2024,(09):.  
基于Wnt/β-catenin信号通路的健骨伸筋汤对膝骨关节炎患者的干预作用研究
Jiangu Shenjin Decoction Treats Knee Osteoarthritis via Wnt/β-catenin Signaling Pathway
投稿时间:2022-11-09  
DOI:10.3969/j.issn.1673-7202.2024.09.005
中文关键词:  健骨伸筋汤  治疗  Wnt/β-catenin信号通路  β-连环素  膝骨关节炎
English Keywords:Jiangu Shenjin Decoction  Treatment  Wnt/β-catenin signaling pathway  β-Catenin  Knee osteoarthritis
基金项目:国家自然科学基金地区科学基金项目(81860645);海南省自然科学基金面上项目(819MS064);省级重点学科建设项目(SZD1005SC;SZD1005PT)
作者单位
刘洪波,靖春颖,黎正卿,刘彩凤,张熙思,蒲新伟,林日亮 海南医学院中医学院海口571199 
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中文摘要:
      目的:健骨伸筋汤通过Wnt蛋白/β-连环素(Wnt/β-catenin)信号通路对膝骨关节炎(KOA)干预效果的研究。方法:将48只雌性斯泼累格·多雷(SD)大鼠随机分成空白对照组、模型组、美洛昔康片组、抗骨增生片组及健骨伸筋汤中剂量、高剂量组,每组8只。除空白对照组外,其余大鼠均向关节腔注射木瓜蛋白酶进行KOA造模。造模成功后空白对照组与模型组予以正常饮食饮水,其余大鼠分别予以美洛昔康药液、抗骨增生药液及健骨伸筋汤药液灌胃,干预4周。观察大鼠行动程度、膝关节肿胀度;检测血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)炎症介质水平,观察关节软骨病理变化,检测软骨中的β-catenin和Wnt4mRNA表达水平。结果:IL-1β、IL-6和TNF-α的浓度比较,模型组高于空白对照组;美洛昔康组与抗骨增生组比较差异无统计学意义;与模型组比较,用药4组低于模型组;健骨中剂量组低于健骨高剂量组;与健骨中剂量组比较,美洛西康组和抗骨增生组略高于健骨中剂量组,差异无统计学意义;Wnt4与β-catenin的mRNA表达水平:Wnt4模型组表达水平提高,在各给药组水平有所降低;β-catenin的mRNA表达水平,模型组有所降低,在各给药组水平有所提高,健骨伸筋汤骨代谢调节有效。结论:健骨伸筋汤治疗KOA可能是通过Wnt/β-catenin信号通路来抑制炎症介质,调控信号通路内的蛋白来影响骨代谢。
English Summary:
      To study the effect of Jiangu Shenjin Decoction on knee osteoarthritis via the Wnt/β-catenin signaling pathway.Methods:Forty-eight female Sprague-Dawley(SD) rats were randomly assigned into 6 groups(n=8):blank control,model,meloxicam tablets,Kanggu Zengsheng tablets,and medium-and high-dose Jiangu Shenjin Decoction.The rats in other groups except the blank control group were injected with papain in the knee joint cavity for the modeling of knee osteoarthritis.After successful modeling,the blank group and the model group received normal diet and water,and the rats in other groups were administrated with corresponding drugs by gavage for 4 weeks.The degrees of movement and knee swelling were observed.The serum levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α) were measured.The pathological changes in the articular cartilage were observed,and the mRNA levels of β-catenin and Wnt4 in the articular cartilage were examined.Results:The model group had higher levels of IL-1β,IL-6,and TNF-d than the blank control group,and the levels of the three inflammatory mediators had no significant differences between the meloxicam and Kanggu Zengsheng groups.Compared with the model group,the drug treatments lowered the levels of IL-1β,IL-6,and TNF-α.Moreover,the medium-dose Jiangu Shenjin Decoction group had lower levels of IL-1β,IL-6,and TNF-α than the high-dose group.The levels of IL-1β,IL-6,and TNF-α in the meloxicam and Kanggu Zengsheng groups were slightly higher than those in the medium-dose Jiangu Shenjin Decoction group.Compared with the blank control group,the model group showcased up-regulated mRNA level of Wnt4 and down-regulated mRNA level of β-catenin,and such changes were restored by drug treatments.The results indicated that Jiangu Shenjin Decoction was effective in regulating bone metabolism.Conclusion:Jiangu Shenjin Decoction may inhibit inflammatory mediators by regulating the expression of proteins in the Wnt/β-catenin signaling pathway,thereby influencing bone metabolism.
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