To study the active components and mechanism of anthocyanins from Prunus cerasifera in Xinjiang(XJP-ACY) in treating atherosclerosis.Methods:The main components were identified by liquid chromatography-mass spectrometry(LC-MS)，and network pharmacology was employed to screen the potential targets of the main components of XJP-ACY and atherosclerosis.A “component-disease-target” network was established to predict the potential signaling pathways involved in the treatment of atherosclerosis with XJP-ACY.The EA.hy926 endothelial cell model of cell injury was established with oxidized low density lipoprotein(ox-LDL) and treated with XJP-ACY.The effects of XJP-ACY on the viability，activities of antioxidant enzymes，apoptosis，and adhesion of the cells were examined.Western blotting was employed to determine the expression of related proteins，on the basis of which the prediction results of network pharmacology were validated.Results:Four components and 21 potential antiatherogenic targets of XJP-ACY were predicted.Two gene clusters and two core genes(FGF2 and ADRA2C) were yielded by the cluster analysis.The gene ontology analysis results showed that the selected targets were mainly involved in biological processes such as inflammatory response and positive regulation of MAPK.The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed 28 signaling pathways，including the PI3K-AKT signaling pathway，AGE-RAGE signaling pathway in diabetic complications，and C-type lectin receptor signaling pathway.The cell experiment results showed that the suitable modeling conditions were incubation with 100 μmol/L ox-LDL for 24 h.XJP-ACY increased the survival rate，elevated the nitric oxide and superoxide dismutase levels，lowered the lactate dehydrogenase，malondialdehyde，endothelin-1，and reactive oxygen species levels，inhibited intercellular adhesion molecule-1 expression，and reduced inflammatory cytokine secretion in the EA.hy926 cells treated with ox-LDL.In addition，XJP-ACY inhibited the apoptosis of EA.hy926 cells exposed to ox-LDL by upregulating the expression of B-cell lymphoma-2.Conclusion:XJP-ACY can treat atherosclerosis by inhibiting inflammation and promoting apoptosis in a multi-component，multi-target，and multi-pathway manner.