世界中医药

基金项目:国家自然科学基金地区科学基金项目(81760847，82060844）；广西自然科学基金重点项目(2018GXNSFDA050018）；广西自然科学基金项目（2022GXNSFBA035663）；广西中医药大学一流学科开放课题（2019XK018）；2020年广西高等学校高水平创新团队及卓越学者计划（桂教人才〔2020〕6号）；广西中医脑病临床研究中心（桂科AD20238028）；广西中医药重点学科建设项目（GZXK-Z-20-13）

作者	单位
陈炜1，2，匡龙娇3，胡跃强2，吴林4，朱健敏3，袁炳茂3，洪煌钟3，朱小敏3，蒋凌飞3	1 江西中医药大学研究生院，南昌，330004； 2 广西中医药大学第一附属医院脑病科，南宁，530023； 3 广西中医药大学研究生院，南宁，530001； 4 广西中医药大学科学实验中心，南宁，530001

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中文摘要:

目的：探讨益肺宣肺降浊方及通过影响脑源性神经营养因子(BDNF)改善血管痴呆记忆的作用机制。方法：将Wistar大鼠随机分为假手术组，模型组，益肺宣肺降浊方低剂量组，益肺宣肺降浊方中剂量组，益肺宣肺降浊方高剂量组，石杉碱甲组，每组15只。其中除假手术组外均构建大脑中动脉栓塞法(MCAO）大鼠血管性痴呆模型。益肺宣肺降浊方低剂量组、中剂量组、高剂量组分别给予1.25、2.5、5 g/kg的益肺宣肺降浊方灌胃，假手术组和模型组给予同等体积的生理盐水灌胃，石杉碱甲组给予3 mg/kg的石杉碱甲灌胃。各组每日灌胃1次，持续给药4周后，进行Morris水迷宫试验检测动物神经行为学；Morris水迷宫结束后，获取大鼠海马组织，通过TUNEL染色检测海马组织细胞凋亡情况；用免疫组织化学检测海马组织中BDNF表达。结果：Morris水迷宫行为学结果显示，与模型组比较，不同剂量的益肺宣肺降浊方组的逃避潜伏期显著减少（P<0.05），穿越平台次数明显增加(P<0.05)；TUNEL结果表明益肺宣肺降浊方可有效抑制海马神经元凋亡（P<0.05）；免疫组织化学结果显示益肺宣肺降浊方显著上调大鼠海马组织中BDNF的表达（P<0.05）。结论：益肺宣肺降浊方可改善血管痴呆大鼠的学习和记忆功能障碍，其作用机制与抑制神经元凋亡和上调BDNF表达有关。

English Summary:

To investigate the role and mechanism of Yifei Xuanfei Jiangzhuo Formula in improving memory in vascular dementia by regulating brain-derived neurotrophic factor(BDNF).Methods:Wistar rats were randomly assigned into sham，model，and low-，medium-，high-dose(1.25，2.5，and 5 g/kg，respectively) Yifei Xuanfei Jiangzhuo Formula，and huperzine A(3 mg/kg) groups(n=15).The rats in other groups except the sham group were subjected to middle cerebral artery occlusion(MCAO) for the modeling of vascular dementia.The rats in the drug intervention groups were administrated with corresponding drugs by gavage，and those in the sham and model groups were administrated with the same volume of normal saline once a day for 4 weeks.Morris water maze test was performed to examine the neurobehaviors of the rats.After the test，the hippocampal tissue was collected，and the terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) was employed to detect apoptosis in the hippocampal tissue.The BDNF expression in the hippocampal tissue was determined by immunohistochemistry.Results:The results of Morris water maze test showed that compared with the model group，Yifei Xuanfei Jiangzhuo Formula shortened the escape latency(P<0.05) and increased the number of crossing the platform(P<0.05).The TUNEL results showed that Yifei Xuanfei Jiangzhuo Formula inhibited the apoptosis of hippocampal neurons(P<0.05).The immunohistochemical results showed that Yifei Xuanfei Jiangzhuo Formula up-regulated the BDNF expression in the hippocampal tissue(P<0.05).Conclusion:Yifei Xuanfei Jiangzhuo Formula can improve the learning and memory in the rat model of vascular dementia by inhibiting neuronal apoptosis and up-regulating BDNF expression.

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