| 引用本文:冯丹1，丑丹1，张妍2，蔡静2.补肾活血汤调控宫腔粘连大鼠内膜纤维化进程的作用机制研究[J].世界中医药,2024,(12):. |
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| 补肾活血汤调控宫腔粘连大鼠内膜纤维化进程的作用机制研究 |
| Bushen Huoxue Decoction Regulates Endometrial Fibrosis in Rat Model of Intrauterine Adhesions |
| 投稿时间：2023-12-13 |
| DOI：10.3969/j.issn.1673-7202.2024.12.007 |
| 中文关键词: 宫腔粘连 微RNA-655 冷诱导核糖核酸结合蛋白 补肾活血汤 转化生长因子β1 雌二醇 肿瘤坏死因子-α |
| English Keywords:Intrauterine adhesion microRNA-665 Cold-inducible RNA-binding protein Bushen Huoxue Decoction Transforming growth factor-β1 Estradiol Tumor necrosis factor-α |
| 基金项目:国家自然科学基金青年科学基金项目（82274077）；湖北省卫健委重点项目（MY2021M019） |
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| 中文摘要: |
| 目的：探究微RNA-655（miR-665）/冷诱导核糖核酸结合蛋白（CIRBP）在宫腔粘连（IUA）子宫内膜纤维化中的作用以及补肾活血汤对其的调控作用。方法：使用miRNA靶基因数据库（TargetScan）分析miR-665和CIRBP的结合情况；通过实时定量聚合酶链反应（qRT-PCR）和蛋白免疫印迹技术检测细胞和动物模型中miR-665和CIRBP的转录和表达水平；借助双荧光素酶报告系统和构建过表达载体验证miR-665和CIRBP之间相互调控的作用，以及补肾活血汤对miR-665/CIRBP的调控作用；利用酶联免疫吸附测定（ELISA）和苏木精-伊红（HE）染色检测大鼠宫腔粘连模型中相关因子水平和组织病变程度。结果：在宫腔粘连模型大鼠组织中，CIRBP转录水平显著上升，而miR-665转录水平显著下降。体外实验显示，miR-665模拟物可降低子宫内膜基质细胞中CIRBP的表达水平。此外，补肾活血汤能够降低宫腔粘连的大鼠模型血清中转化生长因子β1（TGF-β1）、雌二醇（E2）和肿瘤坏死因子-α（TNF-α）的水平。补肾活血汤可以提高miR-665的转录水平并降低CIRBP的表达水平，并减轻大鼠子宫内膜组织的病变程度。结论：补肾活血汤通过调控miR-665/CIRBP途径来减轻宫腔粘连大鼠子宫内膜纤维化程度，为宫腔粘连的治疗提供了新的策略和方向。 |
| English Summary: |
| To investigate the role of microRNA-665(miR-665)/cold-inducible RNA-binding protein(CIRBP) in endometrial fibrosis in intrauterine adhesion(IUA) and the regulatory effect of Bushen Huoxue Decoction.Methods:TargetScan was used to analyze the binding of CIRBP with miR-665.Quantitative real-time PCR and Western blotting were employed to determine the transcription and expression levels of CIRBP and miR-665 in cells and animal models.The dual-luciferase reporter system and overexpression vector were utilized to examine the mutual regulation between CIRBP and miR-665，as well as the regulatory effects of Bushen Huoxue Decoction on miR-665/CIRBP.Enzyme-linked immunosorbent assay and hematoxylin-eosin staining were performed to measure the levels of relevant factors and pathological changes in the rat model of IUA.Results:The rat model of IUA showed upregulated transcription level of CIRBP and downregulated transcription level of miR-665.The in vitro experiments showed that miR-665 mimics downregulated the expression level of CIRBP in endometrial stromal cells.Bushen Huoxue Decoction lowered the levels of transforming growth factor-β1(TGF-β1)，estradiol(E2)，and tumor necrosis factor-α(TNF-α) in the serum of the rat model.Additionally，Bushen Huoxue Decoction upregulated the transcription level of miR-665 while downregulating the expression level of CIRBP，thereby alleviating the pathological changes in the endometrial tissue of the rats.Conclusion:Bushen Huoxue Decoction alleviates endometrial fibrosis in the rat model of IUA by regulating the miR-665/CIRBP pathway，which provides new strategies and directions for the treatment of IUA. |
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